Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluid Mosaic Model01:19

Fluid Mosaic Model

11.6K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
11.6K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.0K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.0K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.2K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.2K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

10.9K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10.9K
Membrane Fluidity01:23

Membrane Fluidity

152.0K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
152.0K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The influence of water on the dynamics of alternating polymers P(C<sub>4</sub>EG<sub>4</sub>) and P(C<sub>8</sub>EG<sub>4</sub>) by broadband dielectric spectroscopy.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same author

Backbone Dynamics of Bottlebrush Polymers Studied by Neutron Scattering.

Macromolecules·2026
Same author

Accelerating ion transport by dynamic asymmetry of alternating polymer electrolytes.

Soft matter·2026
Same author

Cyclic Polymers as Nanoscale Platforms for Enzyme Encapsulation and Transport.

ACS applied materials & interfaces·2025
Same author

Importance of end-block contributions in the single chain dynamics of unentangled polymer melts.

Journal of physics. Condensed matter : an Institute of Physics journal·2025
Same author

Dynamics of amphiphilic PEG-PDMS-PEG triblock copolymer assemblies.

Journal of physics. Condensed matter : an Institute of Physics journal·2025

Related Experiment Video

Updated: Jun 21, 2025

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.2K

Ion-Mediated Structural Discontinuities in Phospholipid Vesicles.

Judith U De Mel1, Stefanie Klisch1, Sudipta Gupta1

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, United States.

Langmuir : the ACS Journal of Surfaces and Colloids
|July 9, 2024
PubMed
Summary

Controlling soft matter self-assembly is challenging. Researchers discovered ion-induced changes in phospholipid vesicles, enabling controlled layering of soft materials by manipulating salt concentrations.

More Related Videos

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

14.8K
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
09:29

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Published on: January 19, 2020

8.4K

Related Experiment Videos

Last Updated: Jun 21, 2025

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.2K
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

14.8K
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
09:29

Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration

Published on: January 19, 2020

8.4K

Area of Science:

  • Soft Matter Physics
  • Colloid Science
  • Materials Science

Background:

  • Controlling the self-assembly of soft matter into defined structures is a persistent scientific challenge.
  • Phospholipid vesicles are model systems for studying self-assembly dynamics.

Purpose of the Study:

  • To investigate ion-induced structural changes in phospholipid vesicles for controlled self-assembly.
  • To understand the mechanism behind these structural discontinuities.

Main Methods:

  • Dynamic light scattering
  • Zeta-potential measurement
  • Cryo-electron microscopy
  • Small-angle X-ray scattering
  • Small-angle neutron scattering

Main Results:

  • Observed ion-induced structural discontinuities in DOPC vesicles with varying NaCl concentrations.
  • Identified transitions from unilamellar to bilamellar (at 8 mM NaCl) and trilamellar (at 75 mM NaCl) structures.
  • Demonstrated that declining solvent quality and osmotic pressure drive lipid expulsion and bilayer formation.

Conclusions:

  • Ion-induced structural discontinuities in phospholipid vesicles offer a method for controlled soft material self-assembly.
  • This approach provides a pathway for engineering bioinspired colloidal systems.
  • Understanding these lipid behaviors is key for advanced materials design.