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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex.

Andre M Xavier, Qianyu Lin, Chris J Kang

    Biorxiv : the Preprint Server for Biology
    |July 9, 2024
    PubMed
    Summary

    Sensory experience shapes developing brain circuits by altering gene expression. This study reveals 1,268 sensory-induced genes in mouse visual cortex, highlighting key molecular pathways for neural circuit refinement.

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    Area of Science:

    • Neuroscience
    • Developmental Biology
    • Molecular Biology

    Background:

    • Postnatal brain development relies on sensory experience to refine neural circuits.
    • Molecular mechanisms, particularly sensory-induced gene expression, are crucial but not fully understood.
    • Characterizing developmental gene programs responsive to sensory input is essential for understanding circuit maturation.

    Purpose of the Study:

    • To comprehensively map the transcriptional responses of individual cell types in the developing mouse visual cortex to sensory experience.
    • To identify specific genes and molecular pathways regulated by sensory deprivation and stimulation during a critical developmental period.
    • To provide a valuable single-nucleus RNA sequencing dataset for future research on sensory-guided neural circuit development.

    Main Methods:

    • Single-nucleus RNA sequencing of 118,529 nuclei from mouse visual cortex.
    • Isolation of sixteen distinct neuronal and non-neuronal cell types.
    • Analysis of transcriptional changes in response to sensory deprivation and stimulation using RNA velocity and CellChat.

    Main Results:

    • Identification of 1,268 unique sensory-induced genes in the developing brain.
    • Differential gene expression patterns observed across various cortical cell types, with excitatory neurons (especially layer 2/3 pyramidal neurons) showing high sensitivity.
    • Discovery of Neurexin and Neuregulin signaling networks involved in cell-cell interactions.
    • Evidence that sensory-induced genes in excitatory neurons promote synapse-to-nucleus communication via enhanced kinase activity.

    Conclusions:

    • Sensory experience profoundly influences gene expression programs in specific cell types within the developing visual cortex.
    • The identified sensory-induced genes and signaling pathways offer novel insights into the molecular basis of experience-dependent neural circuit refinement.
    • This dataset serves as a critical resource for dissecting the mechanisms by which sensory input shapes brain wiring during development.