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A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function
Published on: June 2, 2019
Two new pregnane alkaloids from Pachysandra terminalis Sieb. et Zucc. and their cytotoxic activities
Ying Wu1, Yun Rui2, Chao Ding1
1Shaanxi Key Laboratory of Research and Application of Taibai Qi Yao, School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, P.R. China.
Abstract:
Pactermines E and F (1 and 2), two new pregnane alkaloids were isolated from the whole plant of Pachysandra terminalis Sieb. et Zucc. Their structures were determined by physicochemical properties and spectroscopic methods including 1D, 2D NMR, IR, HR-ESI-MS data. Cytotoxic activities against three human cancer A549, HCT116 and SW620 cell lines of the isolated compounds were evaluated by CCK8 method. However, all compounds showed no significant activity against the three cancer cells (IC50>100 μM) except for compound 1, which showed inhibitory effects against HCT116 cells with IC50 values of 84.6 μM.
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