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Circ-ip6k2 Suppresses Tumor Progression By Modulating The Mir-1292-5p/camk2n1 Signal In Clear Cell Renal Cell Carcinoma.

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Circ-ip6k2 Suppresses Tumor Progression By Modulating The Mir-1292-5p/camk2n1 Signal In Clear Cell Renal Cell Carcinoma.

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Circ-IP6K2 suppresses tumor progression by modulating the miR-1292-5p/CAMK2N1 signal in clear cell renal cell

Jian-Ying Tang¹, Lu Yang¹, Qing-Jian Wu²

¹Department of Nephrology, University-Town Hospital of Chongqing Medical University, No 55 road of University-Town, Shapingba District, Chongqing, 401331, P.R. China.

Functional & Integrative Genomics

|July 9, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Circular RNA circ-IP6K2 is downregulated in clear cell renal cell carcinoma (ccRCC), suppressing tumor growth and progression. It acts by sponging miR-1292-5p, impacting the CAMK2N1 tumor suppressor and the β-catenin/c-Myc pathway.

Keywords:

CAMK2N1 Circ-IP6K2 Clear cell renal cell carcinoma Progression miR-1292-5p

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Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy

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Published on: April 29, 2014

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Renal cell carcinoma (RCC), particularly the clear cell subtype (ccRCC), presents a significant clinical challenge due to its aggressive nature and poor prognosis.
Circular RNAs (circRNAs) are emerging as key regulators in tumorigenesis, but their specific roles in ccRCC remain largely undefined.

Purpose of the Study:

To investigate the role of circ-IP6K2 in the development and progression of clear cell renal cell carcinoma.
To elucidate the molecular mechanisms underlying circ-IP6K2's function in ccRCC.

Main Methods:

Analysis of circRNA expression in ccRCC tissues using the GSE100186 dataset.
In vitro assays to assess the effects of circ-IP6K2 on cell proliferation, migration, and invasion.
In vivo xenograft models to evaluate tumor growth inhibition.

Mechanistic studies involving RNA immunoprecipitation and luciferase reporter assays to identify circRNA-miRNA-mRNA interactions.

Main Results:

Circ-IP6K2 was significantly downregulated in ccRCC tissues, with decreased expression correlating with advanced TNM stage, higher histological grade, and poorer overall survival.
Overexpression of circ-IP6K2 suppressed ccRCC cell proliferation, migration, and invasion in vitro, and inhibited tumor growth in vivo.
Circ-IP6K2 functioned as a molecular sponge for miR-1292-5p, leading to the upregulation of its target gene, CAMK2N1.
CAMK2N1, a tumor suppressor, negatively regulated the oncogenic β-catenin/c-Myc signaling pathway.

Conclusions:

Circ-IP6K2 acts as a tumor suppressor in ccRCC by modulating the miR-1292-5p/CAMK2N1 axis and inhibiting the β-catenin/c-Myc pathway.
These findings highlight circ-IP6K2 as a potential therapeutic target for ccRCC treatment.
The study provides novel insights into the molecular mechanisms driving ccRCC progression.