Circulating circ_0069094 is Correlated with the Present and Endothelial Injury of Acute Coronary Syndrome

Area of Science:

• Cardiovascular Biology
• Molecular Biology
• Biochemistry

Background:

• Acute coronary syndrome (ACS) is a major cause of mortality worldwide.
• CircRNAs are increasingly recognized for their roles in various diseases, including cardiovascular conditions.
• Understanding the specific roles of circRNAs like circ_0069094 in ACS pathogenesis is crucial for developing novel diagnostic and therapeutic strategies.

Purpose of the Study:

• To investigate the expression and functional role of circ_0069094 in acute coronary syndrome (ACS).
• To explore the diagnostic value of circ_0069094 in ACS.
• To elucidate the underlying molecular mechanism of circ_0069094 in ACS, including its interaction with miR-484.

Main Methods:

• Real-time polymerase chain reaction (RT-PCR) for circ_0069094 expression analysis.
• Receiver Operating Characteristic (ROC) curve analysis for diagnostic performance evaluation.
• Assessment of biochemical markers (SOD, MDA, vWF) and cardiac function parameters (LVIDs, LVDD, LVEF) in ACS rat models.
• Cell viability (CCK-8), migration (Transwell), and apoptosis (flow cytometry) assays in H/R HUVEC models.
• Bioinformatic analysis (GO, KEGG) to identify targeted genes of miR-484.
• Correlation analysis using Spearman's method.

Main Results:

• Circ_0069094 concentration was significantly decreased in patients with ACS, ACS rat models, and H/R HUVEC models.
• Increased circ_0069094 expression ameliorated the dysfunction of SOD, MDA, vWF, LVIDs, LVDD, and LVEF in ACS models.
• Circ_0069094 positively regulated the viability, migration, and apoptosis of H/R cell models.
• MiR-484 was identified as a competing endogenous RNA (ceRNA) of circ_0069094, mediating its functional effects.

Conclusions:

• Circ_0069094 acts as a protective factor in acute coronary syndrome.
• Circ_0069094 exhibits potential as a diagnostic biomarker for ACS.
• The circ_0069094/miR-484 axis plays a critical role in the pathogenesis and progression of ACS.