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  1. Home
  3. Circulating Tumor Extracellular Vesicles To Monitor Metastatic Prostate Cancer Genomics And Transcriptomic Evolution.
  1. Home
  3. Circulating Tumor Extracellular Vesicles To Monitor Metastatic Prostate Cancer Genomics And Transcriptomic Evolution.

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Circulating tumor extracellular vesicles to monitor metastatic prostate cancer genomics and transcriptomic evolution.

Irene Casanova-Salas1, Daniel Aguilar1, Sarai Cordoba-Terreros1

  • 1Vall d'Hebron Institute of Oncology (VHIO), Vall d'Hebron Barcelona Hospital Campus, Barcelona, Spain.

Cancer Cell
|July 9, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Tumor-derived extracellular vesicles (EVs) in plasma can be profiled for DNA and RNA. This liquid biopsy approach tracks metastatic prostate cancer (mPC) molecular features and treatment responses.

Keywords:
biomarkersextracellular vesiclesliquid biopsyprostate cancertranscriptomics

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Extracellular vesicles (EVs) in plasma are abundant but their multi-omic profiling for tumor interrogation is underexplored.
  • Metastatic prostate cancer (mPC) poses challenges for longitudinal molecular monitoring.

Purpose of the Study:

  • To explore the potential of circulating EVs for multi-omic profiling in mPC.
  • To validate EV-DNA and EV-RNA as biomarkers for mPC progression and treatment response.

Main Methods:

  • Isolation and genomic/transcriptomic profiling of EVs from in vitro/in vivo mPC models and patient plasma.
  • Development of a novel approach (RExCuE) for EV-RNA transcriptomic profiling.
  • Comparison of EV-DNA/RNA profiles with matched patient biopsies and circulating tumor DNA (ctDNA).

Main Results:

  • EV-DNA and EV-RNA contain significant tumor material, validated in mPC patient cohorts.
  • EV-DNA genomic features correlate with patient biopsies, ctDNA, and clinical progression.
  • EV-RNA transcriptomic profiling (RExCuE) captures tumor-associated transcripts and reflects on-therapy adaptation.

Conclusions:

  • EV profiling provides a viable liquid biopsy strategy for longitudinal genomic and transcriptomic analysis of mPC.
  • Circulating EVs can serve as a non-invasive source for monitoring tumor evolution and treatment dynamics.