Hypoglycaemic effect of short acting biosynthetic human and bovine insulin. A comparative study

The aim of the study was to compare the strength of action, the timing of maximal effect and duration of hypoglycaemic effect of biosynthetic human insulin compared to conventional bovine short acting insulin. On that purpose, a modified insulin tolerance test was performed in 10 healthy persons, first with bovine (Kristalni Insulin-Galenika) and a week later, with a biosynthetic human (Bio.-Human Insulin-Lilly) insulin in a dose of 0.15 U/kg body weight. The blood samples for estimation of glycaemia (glucose oxidase method) were collected at 0, 5, 10, 15, 20, 25, 30, 40, 50, 60, 90 and 120 minutes. Comparing the degree of hypoglycaemia, the time of its beginning and its duration, it is concluded that there was no statistically significant difference between biosynthetic human and conventional short acting bovine insulin used in a single dose in healthy persons.