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Comparative transcriptomics analysis on Senecavirus A-infected and non-infected cells
Yan Li1,2, Huanhuan Chu1,3, Yujia Jiang1,4
1College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
Frontiers in Veterinary Science
|July 10, 2024
Summary
Senecavirus A (SVA) infection significantly alters gene expression in host cells, primarily by upregulating transcription. This study reveals SVA
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Senecavirus A (SVA) is an emerging virus causing vesicular disease in pigs, mimicking other serious diseases.
- SVA belongs to the genus Senecavirus, family Picornaviridae, with a positive-sense single-stranded RNA genome.
- Previous work successfully rescued a wild-type SVA from a cDNA clone using reverse genetics.
Purpose of the Study:
- To investigate the transcriptomic changes in host cells following Senecavirus A infection.
- To understand the molecular mechanisms underlying SVA-host interactions.
- To provide a multi-omics perspective on SVA pathogenesis.
Main Methods:
- Inoculation of BSR-T7/5 cells with passage-5 SVA.
- Comparative transcriptomic analysis of infected and non-infected cells at 12 hours post-inoculation.
- Gene Ontology (GO) and KEGG pathway enrichment analyses, alongside SNP, transcription factor, and protein-protein interaction analyses.
Main Results:
- Identified 628 differentially expressed genes, with 565 upregulated and 63 downregulated.
- SVA infection significantly stimulated transcription initiation.
- SVA impacts multiple immunity-related pathways.
Conclusions:
- SVA infection profoundly affects host cell transcription.
- The findings offer insights into SVA's molecular interactions with host cells.
- This study contributes to a comprehensive multi-omics understanding of SVA and its hosts.
