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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2
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Comparative transcriptomics analysis on Senecavirus A-infected and non-infected cells.

Yan Li1,2, Huanhuan Chu1,3, Yujia Jiang1,4

  • 1College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.

Frontiers in Veterinary Science
|July 10, 2024
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Senecavirus A (SVA) infection significantly alters gene expression in host cells, primarily by upregulating transcription. This study reveals SVA

Keywords:
RNA-seqSenecavirus Adifferentially expressed geneenrichment analysisimmunitypathwaytranscriptomics

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Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Senecavirus A (SVA) is an emerging virus causing vesicular disease in pigs, mimicking other serious diseases.
  • SVA belongs to the genus Senecavirus, family Picornaviridae, with a positive-sense single-stranded RNA genome.
  • Previous work successfully rescued a wild-type SVA from a cDNA clone using reverse genetics.

Purpose of the Study:

  • To investigate the transcriptomic changes in host cells following Senecavirus A infection.
  • To understand the molecular mechanisms underlying SVA-host interactions.
  • To provide a multi-omics perspective on SVA pathogenesis.

Main Methods:

  • Inoculation of BSR-T7/5 cells with passage-5 SVA.
  • Comparative transcriptomic analysis of infected and non-infected cells at 12 hours post-inoculation.
  • Gene Ontology (GO) and KEGG pathway enrichment analyses, alongside SNP, transcription factor, and protein-protein interaction analyses.

Main Results:

  • Identified 628 differentially expressed genes, with 565 upregulated and 63 downregulated.
  • SVA infection significantly stimulated transcription initiation.
  • SVA impacts multiple immunity-related pathways.

Conclusions:

  • SVA infection profoundly affects host cell transcription.
  • The findings offer insights into SVA's molecular interactions with host cells.
  • This study contributes to a comprehensive multi-omics understanding of SVA and its hosts.