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  11. X-paste Improves Wound Healing In Diabetes Via Nf-e2-related Factor/ho-1 Signaling Pathway

X-Paste improves wound healing in diabetes via NF-E2-related factor/HO-1 signaling pathway

Ming-Wei Du1,2, Xin-Lin Zhu2, Dong-Xing Zhang3

  • 1Institute of Cardiovascular Disease, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China.

World Journal of Diabetes
|July 10, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

X-Paste (XP) significantly improves diabetic wound healing by activating the Nrf2/HO-1 pathway. Its key component, Andrographolide, protects endothelial cells and reduces inflammation, offering a promising treatment for diabetic foot ulcers.

Area of Science:

  • Biomedical Science
  • Pharmacology
  • Wound Healing Research

Background:

  • Diabetic foot ulcers (DFU) are severe diabetes mellitus (DM) complications, increasing risks of amputation and mortality.
  • Effective therapeutic strategies for DFU remain a critical unmet need.

Purpose of the Study:

  • To investigate the therapeutic mechanisms of X-Paste (XP) for diabetic wound healing.
  • To elucidate the role of XP's key component, Andrographolide (Andro), in regulating cellular processes relevant to DFU.

Main Methods:

  • XP preparation using traditional Chinese medicine methods, component analysis via HPLC.
  • Assessment of XP's efficacy in streptozotocin (STZ)-induced diabetic mouse models.
  • RNA-sequencing for gene expression analysis and molecular docking to identify mechanisms.
Keywords:
AndrographolideDiabetes mellitusNF-E2-related factor-2/HO-1 signaling pathwayWound healing

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  • In vitro studies on human umbilical vein endothelial cells (HUVECs) exposed to high glucose (HG), including Nrf2 knockdown experiments.

    • Main Results:

    • XP significantly accelerated wound healing in diabetic mice.
    • RNA-sequencing identified Nrf2 upregulation in XP-treated tissues; Andro showed strong Nrf2 binding.
    • Andro mitigated HG-induced HUVEC damage, including reduced proliferation, metastasis, and inflammation, via Nrf2/HO-1 pathway activation.

    Conclusions:

    • XP effectively promotes wound healing in diabetic models.
    • Andrographolide is a key active component in XP, activating the Nrf2/HO-1 pathway.
    • Andro enhances endothelial cell proliferation, tubule formation, and reduces inflammation, suggesting therapeutic potential for DFU.