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Anther Culture Protocols for Barley and Wheat
Sue Broughton1, Marieclaire Castello2, Li Liu2
1Department of Primary Industries and Regional Development, South Perth, WA, Australia. Sue.Broughton@dpird.wa.gov.au.
Abstract:
Doubled haploid (DH) techniques remain valuable tools for wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) genetic improvement, and DH populations are used extensively in breeding and research endeavors. Several techniques are available for DH production in wheat and barley. Here, we describe two simple, robust anther culture methods used to produce more than 15,000 DH wheat and barley lines annually in Australia.
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