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A Multienzyme Logic H+ and Na+ Biotransducer.

Yukun Chen1, Mingyin Cui1, Bingfu Liu1

  • 1Graduate School of Information, Production and Systems, Waseda University, 2-7 Hibikino, Wakamatsu, Kitakyushu, Fukuoka 808-0135, Japan.

ACS Applied Materials & Interfaces
|July 10, 2024
PubMed
Summary

This study integrates enzymes into a multienzyme logic system to control sodium and proton concentrations for bioelectronic devices. The system uses chemical inputs to modulate ion levels, enabling advanced biosensor and biotransducer applications.

Keywords:
ATP synthasebiotransducerenzyme logicion modulationmultienzyme

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Area of Science:

  • Bioengineering
  • Enzyme catalysis
  • Bioelectronics

Background:

  • Sodium ions and protons are crucial for cellular and tissue functions.
  • Controlling ion transport is vital for developing effective bioelectronic devices like biosensors.
  • Existing bioelectronic systems require precise ion concentration regulation.

Purpose of the Study:

  • To engineer a multienzyme logic system for regulating proton and sodium ion concentrations.
  • To demonstrate the use of enzyme logic gates for controlling ion levels in bioelectronic applications.
  • To explore advanced on-demand control of biological ions using enzyme-based systems.

Main Methods:

  • Integration of Na+-type ATP synthase, glucose dehydrogenase (GDH), and urease into a multienzyme logic system.
  • Utilizing GDH with glucose and nicotinamide adenine dinucleotide (NAD+) as an AND gate to increase proton concentration.
  • Employing urease to hydrolyze urea as a NOT gate for decreasing proton concentration and resetting the system.
  • Developing a Na+-type ATP synthase-urease system as an AND gate controlled by ADP and urea.

Main Results:

  • A functional multienzyme logic system was created to control proton concentrations using AND and NOT enzyme logic gates.
  • The system successfully modulated local proton and sodium concentrations via chemical input signals.
  • A more complex Na+-type ATP synthase-urease system demonstrated AND gate functionality for ion control.

Conclusions:

  • Multienzyme logic systems offer a novel approach for modulating biologically significant ion concentrations.
  • This research paves the way for advanced, on-demand control in enzyme-based bioelectronic devices.
  • The developed systems show potential for precise regulation in biosensors and biotransducers.