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Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
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Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

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Oxidative stress induces ferroptosis in tendon stem cells by regulating mitophagy through cGAS-STING pathway

Yuanyuan Gao1, Wenshuang Sun2, Junrui Wang3

  • 1Nanjing Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210002, China; Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China.

International Immunopharmacology
|July 10, 2024
PubMed

Insights

Oxidative stress damages tendon stem cells (TSCs) in tendinopathy through ferroptosis. Targeting the cGAS-STING pathway may offer a new therapeutic strategy for this common sports injury.

Area of Science:

  • Orthopedics
  • Cell Biology
  • Biochemistry

Background:

  • Tendinopathy is a common orthopedic sports injury with limited effective treatments.
  • Oxidative stress is known to injure tendon stem cells (TSCs) during tendinopathy, but the underlying mechanisms remain unclear.

Purpose of the Study:

  • To investigate the mechanism of oxidative damage to TSCs in tendinopathy.
  • To explore the role of the cGAS-STING pathway and mitophagy in H2O2-induced TSC injury.
  • To evaluate cGAS as a potential therapeutic target for tendinopathy.

Main Methods:

  • Induction of oxidative damage in TSCs using hydrogen peroxide (H2O2).
  • Assessment of ferroptosis, mitophagy, and the cGAS-STING pathway activation.
  • Inhibition of mitophagy using Mdivi-1 and interference with cGAS function.
  • Evaluation of therapeutic effects in a rat model of tendinopathy.

Main Results:

  • H2O2 induces oxidative damage in TSCs via ferroptosis and increases mitophagy.
  • Inhibiting mitophagy with Mdivi-1 attenuates H2O2-induced ferroptosis.
  • H2O2 activates the cGAS-STING pathway, which regulates mitophagy.
  • Interfering with cGAS impairs mitophagy and ferroptosis, and alleviates tendon injury in rats.

Conclusions:

  • Oxidative stress induces TSC injury in tendinopathy through ferroptosis, modulated by mitophagy and the cGAS-STING pathway.
  • Targeting the cGAS-STING pathway offers a novel therapeutic strategy for tendinopathy treatment.
Keywords:
FerroptosisMitophagyOxidative stressTendinopathyTendon stem cellcGAS-STING

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