Related Experiment Video
Updated: Jun 21, 2025
An Improved Method of RNA Isolation from Loblolly Pine P. taeda L. and Other Conifer Species
Published on: February 22, 2010
High quality RNA extraction protocol for polyphenolics-rich Cotton tissue
Viralkumar B Mandaliya1, Pritesh Bhatt2, Vrinda S Thaker3
1Centre for Advanced Studies in Plant Biotechnology and Genetic Engineering (CPBGE), Department of Biosciences, Saurashtra University, Rajkot, 360 005, Gujarat, India; Department of Microbiology, Faculty of Science, Marwadi University, Rajkot, 360 003, Gujarat, India.
Abstract:
The extraction of high-quality RNA from cotton (Gossypium spp.) is challenging because of the presence of high polyphenolics, polysaccharides, quinones, and other secondary metabolites. A high-throughput RNA extraction protocol is a prerequisite. This Triton-X-100-based RNA extraction method utilizes Polyvinyl pyrrolidone polymer (PVPP) treatment which efficiently removes phenolics, and the application of Lithium chloride (LiCl) has been found that successfully precipitated the high-quality RNA from cotton tissue. Cytoplasmic male sterility (CMS) is a maternally inherited trait associated with specific mitochondrial genome rearrangements or mutations. The suitability of RNA extracted from Cotton CMS lines was assessed. cDNA was synthesized from RNA and assayed for mitochondrial genes (cox3, nad3, nad9) associated with male sterility. This paper discuss the advantages and limitation of this protocol over existing protocol for RNA extraction for polyphenolics-rich plant tissue.
