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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

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Parallel single B cell transcriptomics to elucidate pig B cell repertoire.

Stanley Bram^1,2, Graeme Lindsey¹, Jenny Drnevich³

¹Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S Mathews Avenue, Urbana, IL, 61801, USA.

Scientific Reports

|July 10, 2024

Summary

This summary is machine-generated.

Understanding pig immunology is vital for vaccine development and preventing zoonotic diseases. This study maps the pig B cell repertoire, crucial for advancing swine health and human safety.

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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

Area of Science:

Veterinary Immunology
Comparative Immunology
Infectious Disease Research

Background:

Swine health is critical globally due to pork consumption and zoonotic disease risks.
Pigs act as viral "mixing vessels," potentially leading to novel human-infecting viruses.
Current swine vaccine development is hindered by complex infections and limited understanding of pig immune responses.

Purpose of the Study:

To comprehensively map the pig B cell repertoire.
To develop methods for analyzing swine adaptive immune responses to pathogens.
To provide insights for improved swine vaccines and zoonosis prediction.

Main Methods:

Developed methods for parallel global and immunoglobulin transcriptome sequencing of up to 10,000 single pig B cells.
Adapted existing computational pipelines for human/mouse sequences to analyze pig B cell data.
Enabled comprehensive mapping of the B cell repertoire and retrieval of paired antibody sequences.

Main Results:

Successfully mapped the pig B cell repertoire using high-throughput sequencing.
Obtained paired antibody sequences from pigs in a single experimental run.
Established a robust computational pipeline for swine B cell repertoire analysis.

Conclusions:

The developed methods offer significant implications for understanding swine diseases and zoonosis.
This research is crucial for advancing swine and human vaccine development.
Mapping the B cell repertoire provides insights for new diagnostic agents and disease control strategies.