Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

7.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.8K
Differential Staining Technique01:26

Differential Staining Technique

2.7K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Foundation for Advancing Studies of the Biodegradation of Polyethylene Surrogates by Environmental and Model Laboratory Microbes.

Environmental microbiology reports·2026
Same author

Systemic Manifestations and Mortality Risk in Transthyretin V142I Variant Carriers: A Million Veteran Program Analysis.

JACC. CardioOncology·2026
Same author

Risk of Systemic Manifestations in Homozygous Carriers of the Transthyretin V142I Variant: A Million Veteran Program Analysis.

Journal of the American Heart Association·2025
Same author

Azotobacter vinelandii as a Nitrogen-Negative Chassis for Bio-Oil and Bio-Wax Production of Heterologous and Native Lipids.

MicrobiologyOpen·2025
Same author

Epidemiology of Metastatic Castration-Resistant Prostate Cancer in Veterans Nationwide.

Journal of the National Comprehensive Cancer Network : JNCCN·2025
Same author

Enhanced Urea Production in the Diazotroph Azotobacter vinelandii as a Means of Stable Nitrogen Biofertiliser Production.

Microbial biotechnology·2025

Related Experiment Video

Updated: May 5, 2026

piggyBac Transposon System Modification of Primary Human T Cells
10:02

piggyBac Transposon System Modification of Primary Human T Cells

Published on: November 5, 2012

17.3K

A deoxyviolacein-based transposon insertion vector for pigmented tracer studies.

Benjamin R Dietz1, Tyler J Nelson2, Neil E Olszewski2

  • 1Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, Minnesota, USA.

Microbiologyopen
|July 11, 2024
PubMed
Summary

Researchers developed a new transposon system for bacteria to produce the pigment deoxyviolacein. This tool helps identify and track specific microbial strains in complex environments.

Keywords:
deoxyviolaceinendophytepigment productionscreeningtransposon

More Related Videos

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System
07:04

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Published on: February 4, 2021

2.0K
Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy
09:46

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Published on: February 26, 2021

3.2K

Related Experiment Videos

Last Updated: May 5, 2026

piggyBac Transposon System Modification of Primary Human T Cells
10:02

piggyBac Transposon System Modification of Primary Human T Cells

Published on: November 5, 2012

17.3K
Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System
07:04

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Published on: February 4, 2021

2.0K
Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy
09:46

Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy

Published on: February 26, 2021

3.2K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Pigment production is a valuable tool for visually identifying and quantifying specific microbial strains within complex communities.
  • Predicting and controlling pigment production levels can be challenging due to intrinsic microbial factors affecting gene expression.

Purpose of the Study:

  • To develop a versatile transposon system for random genomic insertion of deoxyviolacein production genes.
  • To enable precise control over pigment expression for reliable microbial strain identification and tracking.

Main Methods:

  • Constructed a transposon system incorporating deoxyviolacein biosynthesis genes.
  • Randomly inserted the transposon system into bacterial genomes to identify optimal pigment production sites.
  • Applied the system to model bacteria and endophytes for strain differentiation and environmental tracking.

Main Results:

  • Successfully differentiated pigment-tagged strains from complex microbial communities.
  • Confirmed the persistence of tagged strains in natural environments for extended periods.
  • Demonstrated applications in tracing bacterial strains in plant tissues and developing reporter strains for environmental sensing.

Conclusions:

  • The developed transposon system offers a robust method for generating pigment-producing microbial strains.
  • This tool enhances the ability to track and identify bacteria in diverse ecological and experimental settings.
  • The methodology is adaptable for broader applications across various microbes and research objectives.