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Updated: Jun 21, 2025
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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
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MCM8 promotes gastric cancer progression through RPS15A and predicts poor prognosis
Lixian Ding1,2, Mingjun Sun1,2, Yanyan Sun1,2
1Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
Cancer Medicine
|July 11, 2024
Summary
Minichromosome maintenance protein 8 (MCM8) is upregulated in gastric cancer (GC), predicting poor prognosis. MCM8, along with RPS15A, drives GC progression by promoting proliferation and invasion.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gastric cancer (GC) is a leading cause of cancer death globally.
- Minichromosome maintenance protein 8 (MCM8) plays a role in DNA repair and replication.
- MCM8 exhibits tumor-promoting functions in digestive cancers and is a potential therapeutic target.
Purpose of the Study:
- To investigate the expression and clinicopathological significance of MCM8 in GC.
- To elucidate the functional role of MCM8 in GC progression.
- To identify the molecular targets of MCM8 in GC.
Main Methods:
- Bioinformatic analysis of MCM8 expression and clinical data.
- Immunohistochemistry (IHC) and qRT-PCR for MCM8 detection.
- In vitro assays (cell counting, colony formation, migration, invasion, apoptosis) to assess MCM8 function.
- Gene expression microarrays and phospho-kinase arrays to identify MCM8 targets and signaling pathways.
- In vivo studies using a xenograft mouse model.
Main Results:
- MCM8 was significantly upregulated in GC and correlated with poor prognosis, advanced stage, and lymph node metastasis.
- MCM8 knockdown inhibited GC cell proliferation, migration, and invasion, while promoting apoptosis.
- RPS15A was identified as a direct target of MCM8, and the MCM8/RPS15A complex promoted phosphorylation of P38α, LYN, and p70S6K.
- In vivo, MCM8 knockdown suppressed tumor growth and related signaling pathways.
Conclusions:
- MCM8 acts as an oncogene in GC, predicting unfavorable outcomes.
- The MCM8/RPS15A axis is crucial for GC progression.
- Targeting MCM8 may offer a therapeutic strategy for GC.
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