Development of an In Situ Hybridization Method for Detection of Akabane Virus
Nihat Toplu1, Tuba Çiğdem Oğuzoğlu2, Ayşe Nur Akkoç1
1Department of Pathology, Faculty of Veterinary Medicine, University of Aydın Adnan Menderes, Isikli, Aydin, Turkiye.
Abstract:
Akabane virus (AKAV) is an arbovirus belonging to the family Bunyaviridae, genus Orthobunyavirus. AKAV consists of three-segment (L, M, and S RNA segments), negative single-stranded RNA. The aim of this study was to investigate an in situ hybridization method (ISH) in a Vero E6 cell line infected with Akabane virus. The 320 base pair amplicon was obtained by RT-PCR with a primer pair and labeled with digoxigenin. Akabane virus RNAs were seen as a granular pattern in the cytoplasm of infected cells. As a result, the expression of the particular Akabane virus gene area was successfully disclosed in the current investigation using the ISH method with a digoxigenin-labeled probe.


