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Multikingdom oral microbiome interactions in early-onset cryptogenic ischemic stroke.
Muhammed Manzoor1, Jaakko Leskelä1, Milla Pietiäinen1,2
1Department of Oral and Maxillofacial Diseases, University of Helsinki, 00014 Helsinki, Finland.
ISME Communications
|July 11, 2024
Summary
The oral microbiome, including bacteria, viruses, and fungi, differs significantly in young patients with cryptogenic ischemic stroke (CIS). These findings suggest complex multikingdom microbial interactions may influence stroke development.
Area of Science:
- Microbiology
- Neurology
- Genomics
Background:
- The oral microbiome's role in ischemic stroke is under investigation.
- Limited understanding exists regarding multikingdom microbial interactions in cryptogenic ischemic stroke (CIS).
Purpose of the Study:
- To investigate differences in oral microbiota composition and function between young CIS patients and controls.
- To explore multikingdom microbial interactions in the context of CIS.
Main Methods:
- Prospective, multicentre case-control study with 308 participants.
- Saliva metagenomic analysis to identify bacterial, viral, and fungal species.
- Analysis of Compositions of Microbiomes with Bias Correction (ANCOM-BC2) and HUMANn3 for differential abundance and functional potential.
Main Results:
- Significant differences in oral microbiota composition and functional capacity were observed in CIS patients.
- Fifty-one microbial species, including 47 bacterial, 3 viral, and 1 fungal species, were associated with CIS.
- Co-abundance network analysis revealed a more intricate microbial network in CIS patients, suggesting cross-kingdom interactions.
Conclusions:
- The oral microbiome exhibits distinct characteristics in young CIS patients.
- Multikingdom microbial interactions within the oral cavity may contribute to the pathogenesis of cryptogenic ischemic stroke.
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