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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: May 12, 2026

Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
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Single-Cell Synchro-Subtractive-Additive Nanoscale Surgery with Femtosecond Lasers.

Shuyuan Qu1,2, Chenqi Yi3, Qin Zhao1,2

  • 1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan 430079, China.

Nano Letters
|July 11, 2024
PubMed
Summary

Femtosecond laser single-cell surgery (FLSS) precisely manipulates cells using 3D additive and subtractive manufacturing. This minimally invasive technique enables new synthetic biology approaches and cell modification.

Keywords:
direct writingfemtosecond laserlasernanoscale surgerysingle-cell

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Nanotechnology

Background:

  • Existing single-cell manipulation techniques have limitations.
  • Precise modification of cellular structures is challenging.

Purpose of the Study:

  • To introduce a novel in situ "synchro-subtractive-additive" technique for femtosecond laser single-cell surgery (FLSS).
  • To overcome the inadequacies of current methods for single-cell manipulation.

Main Methods:

  • Utilizing synchronized nanoscale three-dimensional (3D) subtractive and additive manufacturing.
  • Employing nonthermal ablation for precise removal and modification of cell structures.
  • Achieving ultrafast solidification of hydrogels via dual photopolymerization.

Main Results:

  • FLSS demonstrated a minimally invasive approach with a 70% post-operative survival rate.
  • The technique supports stable cell proliferation post-surgery.
  • Successful modification of cellular structures and physiological activities was achieved.

Conclusions:

  • FLSS offers a precise and minimally invasive method for single-cell surgery.
  • This technique advances bottom-up synthetic biology.
  • FLSS provides new avenues for creating synthetic organelle-like structures and modifying cell functions.