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Updated: Jun 21, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition
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Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

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LncRNA MEG3 Inhibits the Epithelial-mesenchymal Transition of Bladder Cancer Cells through the Snail/E-cadherin Axis

Liang Wang1, Ping Wang2, Bing Liu3

  • 1Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Current Medical Science
|July 11, 2024
PubMed
Abstract

Insights

Maternally expressed gene 3 (MEG3) long noncoding RNA inhibits bladder cancer cell invasion and migration. MEG3 suppresses epithelial-mesenchymal transition (EMT) by targeting Snail, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Bladder cancer is a significant health concern.
  • Epithelial-mesenchymal transition (EMT) is a key process in cancer progression and metastasis.
  • Long noncoding RNAs (lncRNAs) are emerging as critical regulators in various cancers.

Purpose of the Study:

  • To investigate the role of maternally expressed gene 3 (MEG3) lncRNA in bladder cancer.
  • To elucidate the mechanisms by which MEG3 affects epithelial-mesenchymal transition (EMT) in bladder cancer cells.

Main Methods:

  • Assessed cell invasion, migration, and wound healing.
  • Measured E-cadherin expression via Western blotting, RT-qPCR, and dual luciferase reporter assays.
  • Investigated MEG3 interactions with downstream targets using RNA immunoprecipitation and pull-down assays.

Main Results:

  • MEG3 significantly suppressed bladder cancer cell invasion and migration.
  • MEG3 modulated E-cadherin transcription by binding to the Snail transcription factor.
  • MEG3 inhibited EMT signaling pathways (ERK, JNK, P38), reducing Snail and increasing E-cadherin expression.

Conclusions:

  • MEG3 plays a crucial role in suppressing EMT in bladder cancer.
  • MEG3 demonstrates potential as a therapeutic target for bladder cancer treatment.
Keywords:
Snailbladder cancerepithelial-mesenchymal transitionlong noncoding RNAmaternally expressed gene 3

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