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Related Experiment Video

Updated: Jun 21, 2025

Use of 3D Robotic Ultrasound for In Vivo Analysis of Mouse Kidneys
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Transcutaneous Imaging of Rabbit Kidney Using 3-D Acoustic Wave Sparsely Activated Localization Microscopy With a

Qingyuan Tan, Kai Riemer, Joseph Hansen-Shearer

    IEEE Transactions on Bio-Medical Engineering
    |July 11, 2024
    PubMed
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    This study introduces 3-D acoustic wave sparsely activated localization microscopy (AWSALM) for enhanced micro-vessel imaging. The new method achieves faster, larger field-of-view, super-resolution vascular imaging in vivo.

    Area of Science:

    • Biomedical imaging
    • Ultrasound technology
    • Microvascular research

    Background:

    • Super-resolution ultrasound (SRUS) imaging, or ultrasound localization microscopy (ULM), provides sub-diffraction resolution of micro-vessels by tracking microbubbles.
    • Previous 3-D selective SRUS methods using matrix arrays and phase change contrast agents (PCCAs) were limited by small fields of view (FOV) and complex hardware.

    Purpose of the Study:

    • To develop and validate a novel 3-D acoustic wave sparsely activated localization microscopy (AWSALM) technique.
    • To overcome the limitations of previous SRUS methods by increasing FOV and reducing hardware complexity.

    Main Methods:

    • Proposed 3-D AWSALM utilizing PCCAs and a 128+128 row-column-addressed (RCA) array for ultrafast acquisition.
    • Validated the method on an in-vitro microflow phantom and subsequently in a rabbit kidney in-vivo.

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    Last Updated: Jun 21, 2025

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    Main Results:

    • 3-D AWSALM generated images of a phantom with 8x resolution improvement over the system's point spread function in under 5 seconds.
    • Achieved 3-D microvascular structure, flow speed, and direction super-resolution mapping of a rabbit kidney in under 15 seconds.
    • Demonstrated selective visualization of microvasculature within specific activation volumes and downstream vessels.

    Conclusions:

    • Successfully demonstrated large FOV 3-D AWSALM using an RCA probe.
    • 3-D AWSALM provides a unique in-vivo imaging tool for fast, selective, and large FOV vascular flow mapping.