A transient radial cortical microtubule array primes cell division in Arabidopsis
Isaty Melogno1, Shogo Takatani1,2, Paula Llanos3
1Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon 1, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), CNRS, 69364 Lyon Cedex 07, France.
Summary
Plant cells exhibit variable division plane orientation. A transient radial microtubule pattern before division may ensure cell symmetry, overriding stress alignment.
Area of Science:
- Plant Cell Biology
- Cytoskeleton Dynamics
- Developmental Biology
Background:
- Plant cell division wall formation typically aligns with maximal tensile stress.
- The variability in this process and the role of interphasic microtubules remain unclear.
Purpose of the Study:
- Investigate the origin of variability in plant cell division orientation.
- Determine the role of microtubule behavior before cell division in Arabidopsis stems.
Main Methods:
- Utilized Arabidopsis stems with anisotropic stress patterns.
- Observed cortical microtubule (CMT) organization using live-cell imaging.
- Analyzed microtubule dynamics in wild-type and trm678 mutant cells.
Main Results:
- A specific G2 phase window shows radial CMT organization, preceding preprophase band (PPB) formation.
- This radial pattern correlates with increased cytoplasmic microtubule accumulation.
- Mutant cells lacking PPB show prolonged radial organization and less variable division symmetry despite noisier orientation.
Conclusions:
- The 'radial step' represents a trade-off between division symmetry and orientation robustness.
- Increased cytoplasmic microtubules transiently disrupt CMT alignment with tissue stress during a G2 'reset' stage.
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