Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

3.8K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
3.8K
Synthetic Biology02:55

Synthetic Biology

4.7K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.7K
Antibiotic Selection00:57

Antibiotic Selection

53.0K
Overview
53.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced immunogenicity and dose-sparing efficacy of self-amplifying RNA vaccines against seasonal influenza across subtypes.

Emerging microbes & infections·2026
Same author

Correction to "Structure-Informed Design of High-Cooperativity PROTAC Targeting SARS-CoV-2 RdRp via Click Chemistry and Enhanced Sampling Simulations".

Journal of medicinal chemistry·2026
Same author

Dual-targeting ketolide-quinolone hybrids overcome erm-mediated resistant pathogens via ribosomal and DNA gyrase inhibition.

European journal of medicinal chemistry·2026
Same author

Structure-Informed Design of High-Cooperativity PROTAC Targeting SARS-CoV-2 RdRp via Click Chemistry and Enhanced Sampling Simulations.

Journal of medicinal chemistry·2025
Same author

<i><b>In Silico</b></i> Investigation of BTK as a Potent Immunomodulatory Target: Exploring Novel Therapeutic Approaches for Multiple Sclerosis.

ACS omega·2025
Same author

AI-Accelerated Identification of Novel Antimicrobial Peptides for Inhibiting <i>Fusarium graminearum</i>.

Journal of agricultural and food chemistry·2025

Related Experiment Video

Updated: Jun 21, 2025

Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments
07:50

Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments

Published on: October 25, 2024

1.6K

Synthetic macrolides overcoming MLSBK-resistant pathogens.

Cong-Xuan Ma1, Ye Li2,3, Wen-Tian Liu1

  • 1Key Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China.

Cell Discovery
|July 11, 2024
PubMed
Summary

Novel macrolide-quinolone antibiotics overcome resistance in bacteria like Staphylococcus aureus. These new drugs target methylated ribosomes, offering hope against challenging infections and guiding future antibiotic design.

More Related Videos

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth
09:10

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth

Published on: January 7, 2022

2.3K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.3K

Related Experiment Videos

Last Updated: Jun 21, 2025

Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments
07:50

Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments

Published on: October 25, 2024

1.6K
Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth
09:10

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth

Published on: January 7, 2022

2.3K
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

10.3K

Area of Science:

  • Medicinal Chemistry
  • Microbiology
  • Molecular Biology

Background:

  • Antibiotic resistance, particularly in Staphylococcus aureus, is a growing global health threat.
  • Conventional macrolide-lincosamide-streptogramin B-ketolide (MLSBK) antibiotics are ineffective against bacteria with methylated rRNA (A2058 methylation or G2058 mutation).
  • Existing MLSBK drugs require unmodified A2058 for selective targeting of pathogens over human cells.

Purpose of the Study:

  • To design and synthesize novel macrolide-based antibiotics effective against resistant bacterial strains.
  • To identify compounds that can overcome resistance mechanisms mediated by rRNA methylation.
  • To explore new therapeutic strategies against challenging bacterial pathogens, including Mycoplasma pneumoniae.

Main Methods:

  • Design and synthesis of novel macrolide-quinolone hybrid molecules.
  • Evaluation of antibacterial efficacy against a panel of pathogens, including resistant strains.
  • Mechanistic studies to elucidate the mode of action, focusing on ribosome binding and structure-activity relationships.

Main Results:

  • Discovery of two potent compounds, MCX-219 and MCX-190, with broad-spectrum antibacterial activity.
  • Demonstrated efficacy against A2058-methylated Staphylococcus aureus and clinical Mycoplasma pneumoniae isolates with A2058G mutations.
  • MCX-190 utilizes a secondary binding site in the nascent peptide exit tunnel, engaging methylated ribosomes via specific interactions.

Conclusions:

  • MCX-219 and MCX-190 represent promising next-generation MLSBK antibiotics capable of combating antibiotic resistance.
  • The novel binding mode offers a strategy to overcome resistance conferred by rRNA methylation.
  • These findings provide a framework for the rational design of future MLSBK antibiotics to address escalating resistance challenges.