A genetic variant in the TAPBP gene enhances cervical cancer susceptibility by increasing m6A modification
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Genetic variants influence N6-methyladenosine (m<sup>6</sup>A) levels, impacting cervical cancer (CC) risk. A specific variant, rs1059288 in TAPBP, increases CC susceptibility by altering m<sup>6</sup>A modification, promoting tumor growth and drug resistance.
Area Of Science
- Genetics
- Oncology
- Molecular Biology
Background
- Genetic variants can alter N6-methyladenosine (m<sup>6</sup>A) levels, influencing gene expression and disease susceptibility.
- Understanding the role of m<sup>6</sup>A-associated genetic variants in cervical cancer (CC) is crucial for improving screening and treatment strategies.
Purpose Of The Study
- To identify and characterize m<sup>6</sup>A-associated single nucleotide polymorphisms (SNPs) linked to cervical cancer risk.
- To elucidate the functional and mechanistic role of the identified risk SNP in CC development and progression.
Main Methods
- Genome-wide analysis of m<sup>6</sup>A-associated SNPs using TCGA and JENGER databases with RNA-seq and MeRIP-seq data.
- Case-control study validation of the risk SNP rs1059288 in a cohort of 921 CC cases and 1077 controls.
- In vitro experiments assessing TAPBP's role in CC cell growth, migration, apoptosis, and drug resistance, including pathway analysis.
Main Results
- The SNP rs1059288 (A>G) in the TAPBP 3' UTR was significantly associated with increased CC risk (OR 1.48).
- The risk G allele correlated with elevated m<sup>6</sup>A modification of TAPBP, facilitated by METTL14 and YTHDF2.
- Overexpression of TAPBP in CC tissues promoted tumor growth, migration, and chemoresistance, while inhibiting apoptosis and affecting the JAK/STAT/MICB pathway.
Conclusions
- Genetic variation rs1059288 contributes to cervical cancer susceptibility through altered m<sup>6</sup>A modification of TAPBP.
- TAPBP overexpression drives CC progression and confers drug resistance, highlighting its potential as a therapeutic target.
- Targeting TAPBP or related pathways may offer novel strategies for cervical cancer treatment and prevention.
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
Related Concept Videos
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...