Hsa_circRNA_101036 aggravates hypoxic-induced endoplasmic reticulum stress via the miR-21-3p/TMTC1 axis in oral squamous cell carcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.Hsa_circRNA_101036 acts as a tumor suppressor in oral squamous cell carcinoma (OSCC) by inducing endoplasmic reticulum stress and apoptosis. It regulates TMTC2 expression via sponging miR-21-3p, offering a potential therapeutic target for OSCC.
Area Of Science
- Molecular Biology
- Oncology
- Biochemistry
Background
- Circular RNAs (circRNAs) are emerging as critical regulators in cancer, including oral squamous cell carcinoma (OSCC).
- Hsa_circRNA_101036 has been identified as a potential tumor suppressor in OSCC, but its regulatory mechanisms require elucidation.
- Understanding the role of hsa_circRNA_101036 is crucial for developing novel therapeutic strategies against OSCC.
Purpose Of The Study
- To investigate the regulatory mechanism of hsa_circRNA_101036 in the development and progression of OSCC.
- To explore the potential of hsa_circRNA_101036 as a therapeutic target for OSCC.
- To elucidate the interaction between hsa_circRNA_101036, miR-21-3p, and TMTC2 in OSCC under hypoxic conditions.
Main Methods
- Bioinformatic analysis using StarBase v.2.0 to predict miRNA targets.
- Quantitative assessment of hsa_circRNA_101036, miR-21-3p, and TMTC2 expression in OSCC tissues and cell lines.
- Functional assays including cell viability, migration, invasion, apoptosis, and reactive oxygen species (ROS) analysis following hsa_circRNA_101036 overexpression under hypoxic conditions.
Main Results
- Hsa_circRNA_101036 and TMTC2 expression were downregulated, while miR-21-3p was upregulated in OSCC tissues compared to adjacent tissues.
- Hypoxia significantly decreased hsa_circRNA_101036 and TMTC2 expression while increasing miR-21-3p, HIF-1α, ER stress markers, apoptosis, and ROS levels.
- Overexpression of hsa_circRNA_101036 suppressed tumor growth, induced ER stress, apoptosis, and ROS production, and upregulated TMTC2 by sponging miR-21-3p.
Conclusions
- Hsa_circRNA_101036 functions as a crucial tumor suppressor in OSCC development and progression.
- Hsa_circRNA_101036 exacerbates ER stress, apoptosis, and ROS production in OSCC cells under hypoxic conditions.
- Hsa_circRNA_101036 upregulates TMTC2 by sponging miR-21-3p, highlighting a novel regulatory axis in OSCC.
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