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Nonfermentative bacilli: evaluation of three systems for identification.

L A Otto, U Blachman

    Journal of Clinical Microbiology
    |August 1, 1979
    PubMed
    Summary

    The oxidative attack (OA) system accurately identified nonfermentative bacilli (91%) faster than API 20E and Oxi/Ferm tube systems. While API and Oxi/Ferm are viable alternatives for common isolates, OA excels in both speed and accuracy.

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    Area of Science:

    • Clinical Microbiology
    • Bacteriology
    • Diagnostic Systems Evaluation

    Background:

    • Accurate identification of nonfermentative bacilli is crucial in clinical settings.
    • Existing identification systems vary in performance, necessitating comparative evaluations.
    • The oxidative attack (OA) system offers a non-commercial alternative to kit-based methods like API 20E and Oxi/Ferm tube.

    Purpose of the Study:

    • To evaluate the rapidity and accuracy of three nonfermentative bacilli identification systems: OA, API 20E, and Oxi/Ferm tube.
    • To compare the performance of these systems on 217 clinical isolates.
    • To determine the suitability of each system for routine laboratory use.

    Main Methods:

    • Comparative evaluation of three identification systems (OA, API 20E, Oxi/Ferm tube).
    • Testing on 217 clinical strains of nonfermentative bacilli.
    • Assessment of accuracy and time to identification for each system.

    Main Results:

    • The OA system demonstrated the highest overall accuracy (91%) and speed (98% identification within 48 hours).
    • API 20E (69% accuracy, 50% within 48h) and Oxi/Ferm tube (50% accuracy, 18% within 48h) were less accurate and slower.
    • All systems accurately identified common isolates, but OA outperformed others for fastidious or rare strains.

    Conclusions:

    • The OA system is superior to API 20E and Oxi/Ferm tube in both accuracy and rapidity for identifying nonfermentative bacilli.
    • API 20E and Oxi/Ferm tube may serve as alternatives for common isolates but are significantly inferior to OA when speed is considered.
    • The choice of system depends on the laboratory's needs regarding speed, accuracy, and the types of nonfermentative bacilli typically encountered.

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