Jove
Visualize
Contact Us

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Depletion-Free Automated Enrichment of Serum Glycopeptides for High-Throughput Clinical Glycoproteomics.

Molecular & cellular proteomics : MCP·2026
Same author

RPS6KA3/RSK2-mediated phosphorylation of DRAM2 promotes lysosomal targeting and autophagic flux in melanoma.

Autophagy·2026
Same author

StrucPTM: a database of structurally validated protein modifications and their conformational variation.

Bioinformatics (Oxford, England)·2026
Same author

Non-Reducing Proteomics Reveals Disulfide-dependent Proteoform Remodeling Under Oxidative Stress.

Molecular & cellular proteomics : MCP·2026
Same author

SAPP: Structure Aware PTM Prediction.

Computers in biology and medicine·2025
Same author

Assessing Long-Term Stored Tissues for Multi-Omics Data Quality and Proteogenomics Suitability.

Journal of proteome research·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 21, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.3K

Demystifying PTM Identification Using MODplus: Best Practices and Pitfalls.

Seungjin Na1, Eunok Paek2,3,4

  • 1Digital Omics Research Center, Korea Basic Science Institute, Cheongju, South Korea.

Methods in Molecular Biology (Clifton, N.J.)
|July 12, 2024
PubMed
Summary

This study enhances posttranslational modification (PTM) identification using tandem mass spectrometry (MS/MS). It introduces MODplus software for faster, broader PTM analysis, addressing computational challenges in complex biological samples.

Keywords:
Computational proteomicsMS/MSOpen searchPTMSoftwareTandem mass spectrometryPosttranslational modification

More Related Videos

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.6K
On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
14:15

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

16.5K

Related Experiment Videos

Last Updated: Jun 21, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.3K
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.6K
On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
14:15

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

16.5K

Area of Science:

  • Biochemistry
  • Proteomics
  • Computational Biology

Background:

  • Posttranslational modifications (PTMs) are crucial for regulating biological processes.
  • Tandem mass spectrometry (MS/MS) is a key technology for identifying PTMs.
  • Current methods face challenges in speed and the breadth of detectable PTMs.

Purpose of the Study:

  • To review recent advancements in PTM detection using MS/MS.
  • To address computational challenges in simultaneous multi-PTM searches.
  • To present an automated procedure for extensive PTM identification.

Main Methods:

  • Exploration of advanced MS/MS techniques for PTM discovery.
  • Discussion of computational strategies for complex PTM searches.
  • Introduction and demonstration of the MODplus software for PTM analysis.

Main Results:

  • Recent advancements expand the scope and speed of PTM identification.
  • MODplus provides an automated workflow for comprehensive PTM analysis.
  • A case study illustrates the practical application and results of the MODplus procedure.

Conclusions:

  • The presented methods and MODplus software improve the efficiency and breadth of PTM identification.
  • This work facilitates deeper understanding of biological regulation through PTMs.
  • Automated PTM analysis is crucial for advancing proteomic research.