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Related Concept Videos

Epistasis01:39

Epistasis

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In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
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Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
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Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
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MTHFR as a Novel Candidate Marker for Litter Size in Rabbits.

Jie Yang1, Zhiyuan Bao1, Jiali Li1

  • 1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Animals : an Open Access Journal From MDPI
|July 13, 2024
PubMed
Summary
This summary is machine-generated.

The methylenetetrahydrofolate reductase (MTHFR) gene influences rabbit litter size by affecting granulosa cell function and ovarian follicle development. Specific MTHFR promoter polymorphisms are linked to reproductive performance, offering potential as molecular markers.

Keywords:
MTHFRSNPslitter sizeovarian granulosa cellsrabbits

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Area of Science:

  • Animal Reproduction
  • Molecular Genetics
  • Cell Biology

Background:

  • Litter size is a key economic trait in animal reproduction.
  • The methylenetetrahydrofolate reductase (MTHFR) gene's role in rabbit reproduction requires further investigation.
  • Understanding MTHFR's impact on granulosa cells and follicle development is crucial for improving reproductive efficiency.

Purpose of the Study:

  • To investigate the effect of the MTHFR gene on rabbit granulosa cell apoptosis and proliferation.
  • To explore the association between MTHFR gene polymorphisms and reproductive traits in New Zealand rabbits.
  • To identify the regulatory role of MTHFR in ovarian follicle development.

Main Methods:

  • Flow cytometry and CCK-8 assays were used to assess granulosa cell apoptosis and proliferation.
  • Quantitative reverse transcription PCR (qRT-PCR) and Western blot (WB) analysis were employed to examine gene and protein expression.
  • Dual luciferase reporter assays and Sanger sequencing were utilized to identify the MTHFR promoter region and its polymorphisms.

Main Results:

  • MTHFR influences granulosa cell apoptosis and proliferation, and regulates key follicular development genes (e.g., TIMP1, CITED1, FSHR, GHR).
  • Four single nucleotide polymorphisms (SNPs) were identified in the MTHFR core promoter region.
  • The g.-680C>A polymorphism and its CC genotype were significantly associated with larger total and alive litter sizes in rabbits.

Conclusions:

  • MTHFR plays a significant role in regulating rabbit ovarian granulosa cells and follicle development.
  • Specific MTHFR promoter polymorphisms are linked to variations in litter size.
  • MTHFR holds potential as a molecular marker for enhancing reproductive traits in rabbits.