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A Portable Automated Microfluidic Platform for Point-of-Care Testing for Multiple Mycotoxins in Wine.

Jun Liu1, Shiyu Zeng2, Haoyu Zhu2

  • 1Suqian Product Quality Supervision and Inspection Institute, Suqian 223800, China.

Foods (Basel, Switzerland)
|July 13, 2024
PubMed
Summary

A new portable automated microfluidic platform (PAMP) enables rapid, sensitive point-of-care testing (POCT) for multiple mycotoxins in food. This technology simplifies detection, improving food safety for consumers and the wine industry.

Keywords:
chemiluminescence (CL)mycotoxinspoint-of-care testing (POCT)portable automated microfluidic platform (PAMP)

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Mycotoxins in food pose significant health risks and economic challenges to the wine industry.
  • Traditional detection methods lack the speed, automation, and multiplexing capabilities required for effective point-of-care testing (POCT).

Purpose of the Study:

  • To develop a portable automated microfluidic platform (PAMP) for rapid, sensitive, and simultaneous detection of multiple mycotoxins at the point-of-care.
  • To overcome the limitations of conventional mycotoxin analysis in real-world settings like wineries.

Main Methods:

  • Integration of a chemiluminescence (CL) imaging system with a microfluidic chip utilizing silicone films as substrates.
  • Implementation of streptavidin-biotin (SA-B) system and indirect immunoassay on microfluidic chips for enhanced sensitivity.
  • Development of a PAMP for automated, high-throughput, and quantitative analysis of mycotoxins.

Main Results:

  • Sensitive detection ranges achieved: zearalenone (ZEA) 1-32 ng/mL, aflatoxin B1 (AFB1) 0.2-6.4 ng/mL, and ochratoxin A (OTA) 2-64 ng/mL.
  • High sample recovery rates (91.39-109.14%) demonstrating the reliability and practicality of the PAMP.
  • Significant improvements in detection sensitivity, reduced detection time, and simplified manual operations compared to traditional methods.

Conclusions:

  • The developed PAMP provides a sensitive, rapid, and portable solution for multiple mycotoxin POCT in food safety.
  • This technology is crucial for markets and wineries lacking advanced laboratory facilities, enhancing food safety and consumer protection.
  • The PAMP represents a significant advancement in mycotoxin detection, enabling timely and accurate analysis in diverse settings.