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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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Published on: August 6, 2014

Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay.

David Moreira¹, Daniela Alexandre^1,2, André Miranda¹

¹CICS-UBI-Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, Portugal.

Molecules (Basel, Switzerland)

|July 13, 2024

Summary

This summary is machine-generated.

This study introduces a novel microfluidic assay for detecting miR-155-3p, a key biomarker for lung cancer (LC). The rapid and sensitive molecular beacon assay offers a promising alternative to traditional methods for lung cancer diagnostics.

Keywords:

lung cancer microfluidics mir-155-3p molecular beacon

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Published on: March 14, 2019

Area of Science:

Biomedical Engineering
Molecular Biology
Cancer Research

Background:

Lung cancer (LC) is a leading cause of cancer-related mortality globally.
MicroRNAs (miRNAs) show potential as biomarkers for disease detection and progression, including LC.
Existing miRNA detection methods like PCR are often slow and costly.

Purpose of the Study:

To develop and validate a rapid, sensitive, and cost-effective microfluidic assay for detecting miR-155-3p, a lung cancer biomarker.
To utilize a molecular beacon (MB) bead-based assay integrated into a microfluidic device for enhanced miRNA detection.
To assess the assay's performance in complex biological samples.

Main Methods:

A microfluidic device was employed to immobilize molecular beacon (MB) probes for miRNA detection.
The assay leverages fluorescence enhancement of MBs upon hybridization with the target miR-155-3p.
Performance was evaluated using miR-155-3p in saline, A549 cell total RNA, and spiked peripheral blood mononuclear cells (PBMCs).

Main Results:

The microfluidic MB assay successfully detected miR-155-3p with a limit of detection (LOD) of 42 nM in saline.
Satisfactory recovery rates were achieved when testing the assay with A549 cell total RNA and spiked PBMCs.
The assay demonstrated rapid and straightforward target detection capabilities.

Conclusions:

The developed microfluidic molecular beacon assay provides a promising platform for sensitive and rapid detection of lung cancer-associated miRNAs.
This approach offers a potential alternative to conventional, time-consuming miRNA quantification methods.
Further validation in clinical samples could establish this assay for early lung cancer diagnosis.