Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human B-cell differentiation induced by murein.

J Lüdemann, W L Gross, W Bessler

    Immunology Letters
    |January 1, 1985
    PubMed
    Summary

    Bacterial membrane components can activate B cells to secrete antibodies without T cells. Murein, found in Klebsiella pneumoniae membranes, is identified as the key component responsible for this unique B-cell activation property.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    <i>GripAgain</i> - Development of a Mobile Application (App) for hand function training after nerve surgery.

    Brain & spine·2026
    Same author

    Effectiveness of a home-based training program on hand function after carpal tunnel release: A prospective multicenter trial.

    Brain & spine·2026
    Same author

    A Systematic Review of Workplace Physical Activity Coaching.

    Journal of occupational rehabilitation·2023
    Same author

    Early recovery after surgery for radical cystectomy: comprehensive assessment and meta-analysis of existing protocols.

    World journal of urology·2020
    Same author

    [Primary Prevention in General Medical Practice: A Survey].

    Gesundheitswesen (Bundesverband der Arzte des Offentlichen Gesundheitsdienstes (Germany))·2016
    Same author

    [Daytime flexible application of Insulin degludec in patients with type 1 diabetes or type 2 diabetes].

    MMW Fortschritte der Medizin·2014

    Area of Science:

    • Immunology
    • Microbiology
    • Cell Biology

    Background:

    • Certain bacterial membrane preparations, like Klebsiella pneumoniae (Klebs M), act as polyclonal B-cell activators (PBA) for human B cells.
    • These PBAs induce immunoglobulin secretion without significant prior proliferation or T cell involvement, mimicking 'true' PBAs.
    • The specific biochemical component responsible for this PBA activity within bacterial membranes remained to be identified.

    Purpose of the Study:

    • To identify the specific biochemically defined membrane component possessing polyclonal B-cell activator (PBA) properties.
    • To investigate whether lipoprotein and murein isolated from E. coli could replicate the PBA activity of crude Klebsiella pneumoniae membrane preparations.

    Main Methods:

    • Human mononuclear cells (MNC) and B cell-enriched populations were stimulated with isolated E. coli lipoprotein, murein, and crude Klebsiella pneumoniae membranes (Klebs M).
    • Pokeweed mitogen (PWM) was used as a positive control for proliferation and B-cell differentiation.
    • B-cell activation was assessed by measuring [3H]thymidine incorporation for proliferation and ELISA for secreted immunoglobulin (Ig) production.

    Main Results:

    • Lipoprotein, murein, and Klebs M did not induce significant [3H]thymidine incorporation, indicating a lack of proliferation.
    • PWM significantly stimulated DNA synthesis in MNC.
    • Murein and Klebs M induced B-cell differentiation and Ig secretion in MNC, while lipoprotein did not.
    • In B cell-enriched cultures, only Klebs M and murein induced significant IgM production, suggesting murein is the PBA moiety.
    • Murein induced weaker Ig secretion compared to Klebs M.

    Conclusions:

    • The polyclonal B-cell activator (PBA) moiety within crude Klebsiella pneumoniae membrane preparations is associated with murein.
    • Murein can induce B-cell differentiation and immunoglobulin secretion independently of T cells and significant proliferation.
    • While murein possesses PBA activity, it is less potent than the activity observed with crude bacterial membrane preparations.

    Related Experiment Videos