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Modulation of Bronchial Epithelial Barrier Integrity by Low Molecular Weight Components from Birch Pollen
Srinidhi Sudharson1,2, Tanja Kalic1,2, Julia Eckl-Dorna3
1Department of Dermatology, University Hospital Sankt Poelten, Karl Landsteiner University of Health Sciences, 3100 Sankt Poelten, Austria.
International Journal of Molecular Sciences
|July 13, 2024
Summary
Birch pollen (BP) low molecular weight components (LMC) enhance epithelial barrier function and repair. BP LMC also modulate inflammatory cytokine release, impacting airway epithelial responses.
Area of Science:
- Immunology
- Cell Biology
- Respiratory Medicine
Background:
- Pollen contain low molecular weight components (LMC) below 3 kDa, implicated in allergic responses.
- The specific role of birch pollen (BP)-derived LMC in bronchial epithelial cell interactions remains understudied.
Purpose of the Study:
- To investigate how BP-derived LMC affect bronchial epithelial barrier integrity and function.
- To analyze the impact of BP LMC on epithelial cell cytokine release.
Main Methods:
- Utilized the 16HBE14o- human bronchial epithelial cell line.
- Assessed barrier integrity via transepithelial electrical resistance and xCELLigence system.
- Measured polarized cytokine release using multiplex immunoassay.
Main Results:
- BP LMC significantly enhanced epithelial barrier integrity.
- BP LMC promoted repair of papain-induced epithelial barrier damage.
- Reduced apical release of CCL5 and TNF-α; increased basolateral IL-6 release observed.
Conclusions:
- BP-derived LMC modulate bronchial epithelial cell physical properties and barrier responses.
- LMC play a role in regulating airway epithelial immunological functions.
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