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Related Concept Videos

Skin Cancer01:30

Skin Cancer

4.0K
Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
4.0K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

355
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
355
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

324
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
324

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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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Multi-Wavelength Raman Differentiation of Malignant Skin Neoplasms.

Elena Rimskaya1, Alexey Gorevoy1, Svetlana Shelygina1

  • 1Lebedev Physical Institute, 119991 Moscow, Russia.

International Journal of Molecular Sciences
|July 13, 2024
PubMed
Summary
This summary is machine-generated.

Raman microspectroscopy differentiates skin cancers using spectral features. This technique analyzes molecular differences in healthy, basal cell carcinoma (BCC), and squamous cell carcinoma (SCC) tissues, offering a non-invasive diagnostic tool.

Keywords:
Raman microspectroscopybasal cell carcinomamultispectral analysissquamous cell carcinoma

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Area of Science:

  • Biomedical Optics
  • Molecular Spectroscopy
  • Dermatology

Background:

  • Raman microspectroscopy is a powerful technique for molecular analysis of biological tissues.
  • Distinguishing between healthy and malignant skin tissues, such as basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), is crucial for effective treatment.

Purpose of the Study:

  • To investigate the utility of in vitro Raman spectroscopy for differentiating between healthy, BCC, and SCC skin tissues.
  • To identify specific spectral features and optimal excitation wavelengths for accurate classification.

Main Methods:

  • Acquisition of in vitro Raman spectra from healthy, BCC, and SCC skin tissues using 532 nm and 785 nm laser excitation.
  • Analysis of spectral data in the 900-1800 cm⁻¹ and 2800-3100 cm⁻¹ wavenumber ranges.
  • Evaluation of intensity ratios of specific Raman bands for differentiation.

Main Results:

  • Intensity ratios of bands at 1268, 1336, and 1445 cm⁻¹ at 532 nm effectively differentiated the three sample types.
  • Higher wavenumber bands (2850, 2880, 2930 cm⁻¹) indicated increased protein/lipid ratios in tumors at both wavelengths.
  • Combining data from both excitation wavelengths achieved 87% accuracy for three-class differentiation.

Conclusions:

  • Multi-wavelength Raman spectroscopy offers a versatile, non-invasive method for analyzing molecular changes in malignant skin tumors.
  • This technique shows promise for research into skin cancer processes and potentially other cancers.
  • Optimized spectral analysis can significantly improve diagnostic accuracy for skin cancer detection.