Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microtubule nucleation: How the NEDD1:MZT1:GCP3 trio captures the γ-TuRC.

The Journal of cell biology·2025
Same author

High-resolution yeast actin structures indicate the molecular mechanism of actin filament stiffening by cations.

Communications chemistry·2024
Same author

CryoEM Workflow Acceleration with Feret Signatures.

International journal of molecular sciences·2024
Same author

Harmonizing the Generation and Pre-publication Stewardship of FAIR bioimage data.

ArXiv·2024
Same author

Morphological control enables nanometer-scale dissection of cell-cell signaling complexes.

Nature communications·2022
Same author

Novel cryo-tomography workflow reveals nanometer-scale responses of epithelial cells to matrix stiffness and dimensionality.

Molecular biology of the cell·2022
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2025

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
05:42

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

Published on: July 27, 2022

3.5K

The GoldX Fiducial Eraser.

Peter Van Blerkom1, Armel Bezault2, Cécile Sauvanet3

  • 1Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA 93106, USA.

International Journal of Molecular Sciences
|July 13, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces a new tool to remove artifacts caused by gold nanoparticles used as fiducial markers in cryogenic electron tomography. The method suppresses nanoparticle signals, enhancing tomogram clarity without losing sample data.

Keywords:
cryogenic electron tomographyfiducial markingtomogram processing

More Related Videos

Gold Nanoparticle Synthesis
13:42

Gold Nanoparticle Synthesis

Published on: July 10, 2021

14.5K
Use of Sacrificial Nanoparticles to Remove the Effects of Shot-noise in Contact Holes Fabricated by E-beam Lithography
07:47

Use of Sacrificial Nanoparticles to Remove the Effects of Shot-noise in Contact Holes Fabricated by E-beam Lithography

Published on: February 12, 2017

7.2K

Related Experiment Videos

Last Updated: Jun 21, 2025

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
05:42

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique

Published on: July 27, 2022

3.5K
Gold Nanoparticle Synthesis
13:42

Gold Nanoparticle Synthesis

Published on: July 10, 2021

14.5K
Use of Sacrificial Nanoparticles to Remove the Effects of Shot-noise in Contact Holes Fabricated by E-beam Lithography
07:47

Use of Sacrificial Nanoparticles to Remove the Effects of Shot-noise in Contact Holes Fabricated by E-beam Lithography

Published on: February 12, 2017

7.2K

Area of Science:

  • Cryo-electron tomography
  • Nanoparticle imaging
  • Image processing

Background:

  • Gold nanoparticles (5-15 nm) are standard fiducial markers for alignment in cryogenic electron tomography.
  • High electron density of gold nanoparticles causes artifacts like streaking in tomograms.

Purpose of the Study:

  • To develop an automated tool for detecting and suppressing gold nanoparticle artifacts in cryo-electron tomograms.
  • To improve tomogram quality without compromising biological sample data.

Main Methods:

  • Automated detection of gold nanoparticles within tomographic datasets.
  • Post-processing suppression of detected nanoparticles by replacing them with local background.
  • Validation of the method to ensure no sample information is lost and no new artifacts are introduced.

Main Results:

  • Successful automated detection and suppression of gold nanoparticle artifacts.
  • Generation of cleaner tomograms with reduced streaking.
  • Preservation of relevant cellular or biological sample information.

Conclusions:

  • The developed tool effectively removes gold nanoparticle-induced artifacts in cryo-electron tomography.
  • This post-processing approach enhances tomogram quality for better structural analysis.
  • The method offers a valuable solution for improving imaging in cryo-electron tomography.