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Related Concept Videos

Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

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Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
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Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
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Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

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After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
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Fibril-associated Collagen01:11

Fibril-associated Collagen

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Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
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Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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Anchoring Junctions01:03

Anchoring Junctions

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Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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Related Experiment Video

Updated: Feb 18, 2026

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
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Fibrin-enhanced endothelial cell organization.

J V Olander, M E Bremer, J C Marasa

    Journal of Cellular Physiology
    |October 1, 1985
    PubMed
    Summary

    Adding fibrin significantly speeds up the formation of capillary-like tubes by bovine endothelial cells, from days to hours. This fibrin, along with fibronectin, supports cell organization and may be key to in vivo angiogenesis.

    Area of Science:

    • Cell Biology
    • Biomaterials Science
    • Angiogenesis Research

    Background:

    • Endothelial cell tube formation is crucial for angiogenesis.
    • The role of extracellular matrix components like fibrin in this process requires further elucidation.
    • Previous studies highlight the importance of cell-matrix interactions in vascular development.

    Purpose of the Study:

    • To investigate the effect of fibrin on the in vitro angiogenesis of cloned bovine endothelial cells.
    • To characterize the structural and temporal aspects of fibrin-mediated endothelial tube formation.
    • To assess the relevance of in vitro findings to in vivo angiogenesis.

    Main Methods:

    • Culture of cloned bovine endothelial cells in vitro.
    • Induction of capillary-like tube formation with and without fibrin.

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    Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
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    Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis

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    Last Updated: Feb 18, 2026

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    Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis
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  • Indirect immunofluorescence for fibrin and fibronectin localization.
  • Electron microscopy for ultrastructural analysis and fibrin degradation.

    • Main Results:

    • Fibrin accelerated endothelial cell tube formation from 3-7 days to 2-18 hours.
    • Fibrin and fibronectin were detected along the organized cell strands.
    • Electron microscopy confirmed similar cell structures but showed fibrin degradation within the tube lumen over time.

    Conclusions:

    • Fibrin acts as a potent accelerator for in vitro angiogenesis by bovine endothelial cells.
    • The presence and subsequent degradation of fibrin and fibronectin are integral to the observed cell organization.
    • These in vitro findings suggest a significant role for fibrin in in vivo angiogenesis processes.