Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Facile Surface Modification Strategy for Antibody Immobilization on 3D-Printed Surfaces.

Biosensors·2025

Same author

Optimization of Capillary Vibrating Sharp-Edge Spray Ionization for Native Mass Spectrometry of Triplex DNA.

ACS omega·2025

Same author

Acoustofluidics: Technology Advances and Applications from 2022 to 2024.

Analytical chemistry·2025

Same author

Accessing Different Protein Conformer Ensembles with Tunable Capillary Vibrating Sharp-Edge Spray Ionization.

The journal of physical chemistry. B·2025

Same author

Structure Characterization of a Disordered Peptide Using In-Droplet Hydrogen/Deuterium Exchange Mass Spectrometry and Molecular Dynamics.

ACS physical chemistry Au·2025

Same author

In-droplet hydrogen-deuterium exchange to examine protein/peptide solution conformer heterogeneity.

Rapid communications in mass spectrometry : RCM·2023

Search research articles

Related Experiment Video

Updated: Jun 21, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Nucleic Acid Target Sensing Using a Vibrating Sharp-Tip Capillary and Digital Droplet Loop-Mediated Isothermal

Bethany J Fike¹, Kathrine Curtin^1,2, Peng Li¹

¹C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, USA.

Sensors (Basel, Switzerland)

|July 13, 2024

Summary

This summary is machine-generated.

This study introduces a portable digital nucleic acid test system using a vibrating capillary for precise disease detection at the point-of-care. This innovation enhances quantification accuracy for nucleic acid amplification tests in resource-limited settings.

Keywords:

DNA fragmentation LAMP POC digital detection nucleic acid testing

More Related Videos

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Related Experiment Videos

Last Updated: Jun 21, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Area of Science:

Biotechnology
Molecular Diagnostics
Point-of-Care Testing

Background:

Nucleic acid amplification tests are crucial for disease diagnosis but often require complex equipment.
Isothermal amplification offers a simpler approach for point-of-care (POC) nucleic acid tests.
Current bulk amplification methods lack the quantification accuracy of digital methods.

Purpose of the Study:

To develop a simple, portable system for tunable on-demand droplet generation for digital nucleic acid amplification.
To create a high dynamic range digital droplet loop-mediated isothermal amplification (ddLAMP) system.
To explore the use of vibrating sharp-tip capillary for DNA fragmentation in POC settings.

Main Methods:

Utilized a vibrating sharp-tip capillary for tunable droplet generation.
Integrated droplet generation with loop-mediated isothermal amplification (LAMP) for digital quantification.
Demonstrated DNA fragmentation using modified capillary types with the same mechanism.

Main Results:

Developed a digital droplet loop-mediated isothermal amplification (ddLAMP) system with a wide dynamic range (~2 to 6000 copies/µL).
Achieved tunable on-demand droplet generation using a vibrating sharp-tip capillary.
Showcased the potential for portable DNA fragmentation using the same capillary technology.

Conclusions:

The vibrating sharp-tip capillary enables a simple and portable digital nucleic acid test system.
This technology significantly improves quantification accuracy for POC nucleic acid amplification tests.
The developed system paves the way for accessible digital nucleic acid testing in resource-limited environments.