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Human follicular fluid insulin concentrations.

M P Diamond, B W Webster, R K Carr

The Journal of Clinical Endocrinology and Metabolism

|November 1, 1985

Summary

Insulin in human follicular fluid may play a role in egg maturation. While not directly linked to oocyte maturity or fertilization, insulin levels positively correlated with progesterone, suggesting a physiological function in follicle development.

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Area of Science:

Reproductive Endocrinology
In Vitro Fertilization (IVF)

Background:

Insulin influences granulosa cell activity and steroid production in mammals, impacting oocyte maturation.
Understanding insulin's role in human reproduction is crucial for optimizing fertility treatments.

Purpose of the Study:

To investigate the role of insulin in human follicular and oocyte maturation.
To assess the correlation between follicular fluid insulin levels and various markers of oocyte and follicle development.

Main Methods:

Human follicular fluid was collected 32-36 hours post-hCG administration during IVF procedures.
Follicular fluid insulin levels were quantified using radioimmunoassay (RIA).
Insulin concentrations were analyzed in relation to patient treatment protocols, oocyte maturity, and fertilization rates.

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Main Results:

Follicular fluid insulin levels varied widely (undetectable to 65.4 microU/ml) and did not significantly differ based on stimulation protocols (hMG, clomiphene citrate).
No significant differences in insulin levels were observed between mature and immature oocytes, nor between oocytes that did or did not fertilize.
Follicular fluid insulin levels showed a positive correlation with progesterone levels (P < 0.05) but not with estradiol or androstenedione levels.

Conclusions:

Follicular fluid insulin levels do not appear to be directly associated with oocyte maturity or fertilization success in humans.
The positive correlation between insulin and progesterone suggests a potential physiological role for insulin in human follicular maturation, similar to findings in other mammals.