Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genetic Variations in the P2X7 Receptor: Opportunities and Challenges for Drug Development.

International journal of molecular sciences·2025

Same author

Short-Term Severe Energy Restriction Promotes Molecular Health and Reverses Aging Signatures in Adults With Prediabetes in the PREVIEW Study.

Aging cell·2025

Same author

Clinical response to azacitidine in MDS is associated with distinct DNA methylation changes in HSPCs.

Nature communications·2025

Same author

The CIpP activator, TR-57, is highly effective as a single agent and in combination with venetoclax against CLL cells <i>in vitro</i>.

Leukemia & lymphoma·2024

Same author

The effect of HYPE knock-out on the AMPylome of human OSU-CLL leukemia cells.

Leukemia & lymphoma·2023

Same author

Serum vitamin C status of people in New South Wales: retrospective analysis of findings at a public referral hospital.

The Medical journal of Australia·2023

Search research articles

Related Experiment Video

Updated: Jun 21, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Method for B Cell Receptor Enrichment in Malignant B Cells.

Puja Bhattacharyya^1,2, Richard I Christopherson³, Kristen K Skarratt^1,4

¹Sydney Medical School Nepean, Faculty of Medicine and Health, The University of Sydney, Penrith, NSW 2750, Australia.

|July 13, 2024

Summary

Researchers developed a new method to isolate the B cell receptor (BCR) complex for mass spectrometry analysis. This technique enables comprehensive study of BCR structure and interactions, advancing our understanding of adaptive immunity.

Keywords:

B cell B cell receptor enrichment mass spectrometry

More Related Videos

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Published on: August 13, 2010

Related Experiment Videos

Last Updated: Jun 21, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Induction and Assessment of Class Switch Recombination in Purified Murine B Cells

Published on: August 13, 2010

Area of Science:

Immunology
Proteomics
Biochemistry

Background:

B cells are crucial for adaptive immunity, with B cell receptor (BCR) activation initiating immune responses.
Analyzing the BCR complex via mass spectrometry (MS) is difficult due to challenges in isolating this membrane-bound protein.

Purpose of the Study:

To describe a novel method for enriching the B cell receptor (BCR) complex.
To enable compatibility with mass spectrometry (MS) workflows for structural and interaction analysis.

Main Methods:

Utilized Protein G pull-down on agarose beads with intermediary antibodies targeting BCR subcomponents (CD79a, CD79b, membrane immunoglobulin).
Developed an MS-compatible enrichment strategy for the entire BCR complex.

Main Results:

Successfully enriched the complete BCR complex using the described method.
The technique is compatible with downstream proteomic studies, including MS analysis.
This approach allows for the enrichment of all B cell receptor (BCR) isotypes, overcoming limitations of previous methods.

Conclusions:

The developed method provides a robust approach for BCR complex enrichment.
This technique facilitates advanced proteomic analysis of the BCR, crucial for understanding B cell function and immune responses.
The ability to enrich all BCR isotypes broadens the scope for future research.