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Proinflammatory Activation of Monocytes in Patients with Immunoinflammatory Rheumatic Diseases.
A I Bogatyreva1,2, E V Gerasimova3, T V Kirichenko1
1Avtsyn Research Institute of Human Morphology, Petrovsky Russian Scientific Center of Surgery, Moscow, Russia.
Doklady. Biochemistry and Biophysics
|July 13, 2024
Summary
Monocyte activation in immunoinflammatory rheumatic diseases (IRDs) shows altered cytokine secretion. High basal cytokine levels may impair immune responses, contributing to chronic inflammation in IRDs.
Area of Science:
- Immunology
- Rheumatology
- Cell Biology
Background:
- Immunoinflammatory rheumatic diseases (IRDs) involve chronic inflammation.
- Abnormal macrophage activation is a potential driver of IRD pathogenesis.
- Understanding monocyte behavior is crucial for IRD research.
Purpose of the Study:
- To evaluate the proinflammatory activation of circulating monocytes in patients with IRDs.
- To compare monocyte activation across different IRDs, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and systemic scleroderma (SSc).
Main Methods:
- Primary monocyte cultures were established from 149 participants (53 RA, 45 SLE, 34 SSc, 17 controls).
- Monocytes were assessed for basal and lipopolysaccharide (LPS)-stimulated secretion of TNF-α, IL-1β, and MCP-1 using ELISA.
- Proinflammatory activation was quantified as the ratio of LPS-stimulated to basal cytokine secretion.
Main Results:
- Basal cytokine secretion was elevated in most IRD groups compared to controls.
- LPS-stimulated TNF-α was increased, while MCP-1 was decreased in IRDs.
- Monocyte activation patterns varied, with reduced activation observed in RA, SLE, and SSc patients for specific cytokines.
Conclusions:
- Elevated basal cytokine secretion in IRDs may lead to disrupted immune responses.
- This altered monocyte activation is a significant factor in the pathogenesis of chronic inflammation in rheumatic diseases.
- Findings highlight the complex role of monocytes in IRD progression.
Keywords:
IL-1βMCP-1TNF-αmonocyte activationproinflammatory cytokinesrheumatoid arthritissystemic lupus erythematosussystemic sclerodermaMore Related Videos
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