Bright luminol electrochemiluminescence mediated by a simple TEMPO radical for visualized multiplex detection

In this work, a series of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) radicals bearing different functional groups were exploited as a simple catalyst to promote electrochemiluminescence (ECL) generation in luminol/H₂O₂ system. These TEMPO radicals were found to facilitate the electrochemical oxidation of H₂O₂ and luminol through different catalytic mechanisms, as well as the subsequent ECL generation of luminol/H₂O₂ system. The electrochemical oxidation and luminol ECL generation could be tuned by the functional group on the para-position of TEMPO, for which the structure/activity relationship was revealed. Finally, with the combination of enzymatic system, luminol ECL enhancement up to 9.6-fold was obtained through the catalysis of 4-hydroxyl-TEMPO. The enhanced luminol ECL allows acquiring brighter ECL images in a single-electrochemical system (SEES) for multiplex detection of cholesterol, H₂O₂ and glucose.