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Updated: Jun 21, 2025

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
Published on: May 4, 2015
Methodological approaches to studying phase separation and HIV-1 replication: Current and future perspectives
Andrew J Mouland1, Bao-An Chau1, Vladimir N Uversky2
1Department of Medicine, McGill University, Montreal, Quebec, Canada.
Abstract:
This article reviews tried-and-tested methodologies that have been employed in the first studies on phase separating properties of structural, RNA-binding and catalytic proteins of HIV-1. These are described here to stimulate interest for any who may want to initiate similar studies on virus-mediated liquid-liquid phase separation. Such studies serve to better understand the life cycle and pathogenesis of viruses and open the door to new therapeutics.
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