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Portable loop-mediated isothermal amplification device with spectrometric detection for rapid pathogen

Chun Yu Pan1, Puchong Kijamnajsuk2, Jyh Jian Chen1

  • 1Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, 1, Shuefu Road, Neipu, Pingtung, 91201, Taiwan.

Analytical Biochemistry
|July 13, 2024
PubMed
Summary

A portable loop-mediated isothermal amplification (LAMP) device with spectrometric detection was developed for rapid Salmonella Typhimurium DNA identification. This low-cost, battery-powered tool offers real-time monitoring for improved food safety in remote areas.

Keywords:
Infectious diseasesLoop-mediated isothermal amplification (LAMP)Portable testing platformSalmonella typhimurium DNASpectrometric detection

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Area of Science:

  • Food Safety
  • Microbiology
  • Biotechnology

Background:

  • Increasing global infectious disease spread necessitates rapid, portable diagnostic tools.
  • Resource-limited settings require cost-effective, easy-to-use testing platforms.
  • Existing methods for Salmonella Typhimurium detection can be slow and require specialized equipment.

Purpose of the Study:

  • To develop a portable, low-cost device for real-time detection of Salmonella Typhimurium DNA.
  • To integrate loop-mediated isothermal amplification (LAMP) with spectrometric detection.
  • To create a user-friendly platform for remote monitoring and analysis.

Main Methods:

  • A portable device was constructed using a LinkIt 7697 microcontroller and a microspectrometer.
  • A homemade thermal module with precise temperature control (±1°C) was developed.
  • The device incorporates a thermoelectric cooler, UV LED, and disposable reaction tubes, powered by rechargeable batteries.
  • Real-time spectral signal capture and transmission were enabled for reaction monitoring.

Main Results:

  • The portable LAMP device successfully amplified Salmonella Typhimurium DNA with high sensitivity (2.83 × 10⁻⁴ ng/μL).
  • Accurate temperature control and rapid heating (within 3 min) were achieved.
  • A remote webpage interface facilitated real-time monitoring of temperature and fluorescence.
  • The device demonstrated excellent thermal stability through foam insulation and a 3D-printed frame.

Conclusions:

  • The developed portable LAMP device offers a cost-effective and sensitive solution for Salmonella Typhimurium detection.
  • Real-time monitoring capabilities enhance usability and data analysis for food safety applications.
  • This technology is particularly valuable for resource-limited and non-urban areas, improving food product safety.