Green method for 17-hydroxyprogesterone extraction and determination using PDMS stir bar sorptive extraction coupled...

Area of Science:

• Analytical Chemistry
• Environmental Chemistry
• Biomedical Analysis

Background:

• Accurate quantification of 17-hydroxyprogesterone is essential for various diagnostic and environmental applications.
• Existing methods may lack efficiency, cost-effectiveness, or environmental friendliness for routine analysis.

Purpose of the Study:

• To develop and optimize a cost-effective and green method for quantifying 17-hydroxyprogesterone.
• To extract and analyze 17-hydroxyprogesterone from aqueous and urine matrices using a polydimethylsiloxane stir bar.

Main Methods:

• Solid-phase extraction using a commercial polydimethylsiloxane stir bar.
• High-performance liquid chromatography (HPLC) with UV detection.
• Response surface methodology for optimizing extraction and desorption parameters.

Main Results:

• Optimized method demonstrated high accuracy with predicted relative errors of 1.25% (desorption) and 6.40% (extraction).
• Validated linear ranges were 1.21-1000.00 ng/mL (aqueous) and 2.43-2000.00 ng/mL (urine).
• Low detection limits (0.40 ng/mL aqueous, 0.80 ng/mL urine) and excellent recovery (87-103%) were achieved.

Conclusions:

• The proposed polydimethylsiloxane stir bar extraction method is appropriate, cost-effective, and accurate for 17-hydroxyprogesterone analysis.
• The method's greenness was confirmed through multi-tool assessment, highlighting its environmental benefits.
• This technique provides a reliable approach for routine hormone quantification in diverse samples.