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A method for enumeration ot T and B lymphocytes in tissues.

F O Bastian, P R Middleditch, M I Bossart

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |October 1, 1979
    PubMed
    Summary

    A novel sonication technique isolates viable T and B lymphocytes from tissue. This method allows for easy classification using surface markers, aiding disease evaluation.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biotechnology

    Background:

    • Accurate identification of lymphocyte populations is crucial for diagnosing various diseases.
    • Current methods for lymphocyte isolation can be complex or yield non-viable cells.

    Purpose of the Study:

    • To describe a new sonication technique for isolating T and B lymphocytes from tissue specimens.
    • To establish a reproducible method for lymphocyte identification and classification.

    Main Methods:

    • A sonication technique was employed to process tissue specimens.
    • Isolated lymphocytes were analyzed for viability and purity.
    • Surface markers were used for classification of lymphocyte cell origin.

    Main Results:

    • The sonication method yielded viable and relatively pure lymphocyte populations.
    • Lymphocytes were easily classified based on their distinct surface markers.
    • The technique demonstrated high reproducibility.

    Conclusions:

    • The described sonication method offers an effective means for identifying T and B lymphocytes in tissue.
    • This readily reproducible technique has the potential to become a standard laboratory procedure.
    • It can significantly aid in evaluating disease states requiring lymphocyte origin classification.

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