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Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
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Development of Rapid Disk Diffusion Device Using Laser Speckle Formation Technology for Rapid Antimicrobial

Jaehyeon Lee1,2, Jun Han Lee2, Kyoungman Cho3

  • 1Department of Laboratory Medicine, Jeonbuk National University Medical School and Hospital, Jeonju, Republic of Korea.

Current Microbiology
|July 14, 2024
PubMed
Summary

A new laser speckle formation (LSF) disk diffusion method offers rapid antimicrobial susceptibility testing (AST). This laser-based approach shows potential for faster results compared to conventional methods, aiding in the fight against antimicrobial resistance (AMR).

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Area of Science:

  • Microbiology and Infectious Diseases
  • Biotechnology and Biomedical Engineering

Background:

  • Antimicrobial resistance (AMR) is escalating due to inappropriate antimicrobial use, necessitating faster antimicrobial susceptibility testing (AST).
  • Conventional AST methods (16-24 hours) contribute to empirical prescribing, potentially worsening AMR.
  • There is an urgent need for rapid AST methods to guide effective treatment and combat AMR.

Purpose of the Study:

  • To develop and evaluate a rapid disk diffusion (RDD) method using laser speckle formation (LSF) technology for expedited AST.
  • To assess the performance of LSF technology in determining antimicrobial susceptibility.
  • To compare the novel LSF-based RDD method with conventional disk diffusion (DD) methods.

Main Methods:

  • Development of a unique laser-based rapid disk diffusion (RDD) setup utilizing laser speckle formation (LSF) technology.
  • Preclinical experiments using various bacterial strains to compare LSF-based RDD with conventional disk diffusion (DD).
  • Clinical experiments involving multiple bacterial strains and antibiotics to evaluate LSF technology's performance against DD.

Main Results:

  • Preclinical tests showed over 70% categorical agreement (CA) between LSF-RDD and DD for most antimicrobials.
  • Clinical experiments yielded CA ranging from 40% to 79%, with major and minor discrepancies at approximately 30% and 11%, respectively.
  • High concordance was observed between the laser-based RDD and conventional DD methods across multiple species and antimicrobials.

Conclusions:

  • The developed rapid disk diffusion method based on laser speckle formation technology demonstrates significant potential for hastening AST results.
  • The laser-based RDD method shows higher applicability and potential compared to previously developed rapid AST technologies.
  • This study contributes to the advancement of rapid AST methods, crucial for managing antimicrobial resistance.