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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Jun 21, 2025

Quantitative Analysis of Chromatin Proteomes in Disease

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

Layer-specific proteomic profiling of human normal heart.

Yu Kakimoto¹, Atsushi Ueda¹, Yayoi Kimura²

¹Department of Forensic Medicine, Tokai University School of Medicine, Kanagawa 259-1193, Japan.

Pathology, Research and Practice

|July 14, 2024

Summary

This summary is machine-generated.

This study reveals distinct protein profiles in human heart layers, identifying actin filament-binding proteins that may explain the heart's anisotropic function. This research offers insights into cardiac health and disease.

Keywords:

CTNNA3 Human heart MYH1 Myocardial layers Proteomics Transmural heterogeneity

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Last Updated: Jun 21, 2025

Quantitative Analysis of Chromatin Proteomes in Disease

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

Area of Science:

Cardiovascular Biology
Proteomics
Cardiac Anatomy

Background:

Myocardial layers (inner longitudinal, middle circular, outer longitudinal) are crucial for circulation.
Previous research lacked layer-specific proteomic data for human cardiac tissue.

Purpose of the Study:

To determine the unique proteomic profiles of individual myocardial layers in the human heart.
To investigate the role of these proteins in cardiac function.

Main Methods:

Microdissection of cardiomyocytes from three distinct left ventricular myocardial layers.
Shotgun proteomic profiling and histological analysis.
Immunohistochemical analysis for protein localization.

Main Results:

Identified 1220 proteins, with 92 showing significant layer-specific expression differences.
Proteins elevated in inner and outer layers were predominantly actin filament-binding proteins.
MYH1 and CTNNA3 showed specific high expression in the outer and inner layers, respectively.

Conclusions:

This is the first layer-specific proteomic profile of normal human hearts.
Anisotropic distribution of actin-binding proteins likely contributes to cardiac contractile and conductive anisotropy.
Understanding these profiles aids research into cardiac pathology and treatment, including myocardial infarction.