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Related Concept Videos

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Updated: Jul 20, 2026

Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells
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Long circulating liposomal platform utilizing hydrophilic polymer-based surface modification: preparation,

Jaroslav Turánek1, Petr Kosztyu2, Pavlína Turánek Knötigová3

  • 1ICRC International Clinical Research Center, St. Anne's University Hospital Brno, Czech Republic; Department of Immunology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic; Charles University Prague, Univ. Hosp. Hradec Králové, Inst. Clin. Immunol. & Allergol., Hradec Králové 50005, Czech Republic.

International Journal of Pharmaceutics
|July 14, 2024
PubMed
Summary

N-(2-hydroxypropyl) methacrylamide (HPMA)-based copolymers offer a safer alternative to polyethylene glycol (PEG) for liposome surface modification. These HPMA-modified liposomes demonstrate improved long-term circulation and reduced immune response, enhancing their potential as advanced drug delivery platforms.

Keywords:
Complement activationDrug delivery systemHPMA copolymerLong-circulating liposomesStealth liposomes

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Drug Delivery

Background:

  • Liposomes are crucial drug delivery vectors utilized in clinical settings.
  • Polyethylene glycol (PEG) is commonly used for imparting stealth properties to liposomes.
  • There is a need for improved surface modification agents to enhance liposome performance and safety.

Purpose of the Study:

  • To evaluate N-(2-hydroxypropyl) methacrylamide (HPMA)-based copolymers as surface modifiers for liposomes.
  • To compare the performance of HPMA-modified liposomes with traditional PEGylated liposomes.
  • To assess the biocompatibility and in vivo circulation characteristics of HPMA-modified liposomes.

Main Methods:

  • Liposomes were prepared using lipid film hydration and extrusion through 100 nm polycarbonate filters.
  • Surface modification was achieved using HPMA-based copolymers with cholesterol anchors.
  • Characterization involved transmission electron microscopy, atomic force microscopy, and gradient ultracentrifugation.
  • In vivo circulation studies were conducted in rabbits using fluorescently labeled liposomes.

Main Results:

  • Efficient surface modification of liposomes with HPMA-based copolymers was confirmed.
  • HPMA-modified liposomes exhibited prolonged circulation in vivo compared to PEGylated liposomes.
  • HPMA-modified liposomes did not induce specific antibody formation or complement activation.
  • Repeated administration of HPMA-modified liposomes showed a superior long-circulating effect over PEGylated counterparts.

Conclusions:

  • HPMA-based copolymers are effective and safe surface modifiers for liposomes.
  • HPMA-modified liposomes represent a promising alternative to PEGylated liposomes for drug delivery.
  • These novel liposomal platforms offer enhanced safety and improved long-term circulation for therapeutic applications.