Related Experiment Video
Updated: Jun 21, 2025
10:06
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Published on: April 26, 2017
9.0K
Mapping alternative splicing events in colorectal cancer
Yifeng Zheng1, Guoqiang Zhong1, Qiuyu Song1
1Department of Gastroenterology; Guangdong Provincial Key Laboratory of Major Obstetric Diseases; Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Discover Oncology
|July 14, 2024
Summary
Aberrant alternative splicing (AS) events are implicated in colorectal cancer (CRC). This study identified 14,314 AS events in CRC, revealing specific AS roles in oncogenes like CDK4, offering targets for novel therapies.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- Aberrant gene splicing is linked to colorectal cancer (CRC) development and progression.
- Understanding alternative splicing (AS) event types and their functional impact in CRC is crucial but remains under investigation.
Purpose of the Study:
- To comprehensively map significant alternative splicing (AS) events in colorectal cancer (CRC).
- To investigate the functional roles of identified AS events in key CRC-associated genes and their implications for targeted therapy.
Main Methods:
- Analysis of a public RNA-sequencing database (GSE138202) comparing CRC patients and healthy individuals.
- Identification and characterization of significant alternative splicing (AS) events.
- Functional analysis of AS events in key genes, including splicing factors, microRNAs, and oncogenes.
Main Results:
- Identified 14,314 significant alternative splicing (AS) events in colorectal cancer (CRC) patients.
- Observed diverse AS event types in key CRC-associated genes, including oncogenes.
- Demonstrated that exon 2 skipping in the oncogene CDK4 enhances cell migration and inhibits proliferation in CRC by affecting cell cycle regulation.
Conclusions:
- Alternative splicing (AS) plays a significant role in regulating colorectal cancer (CRC) progression.
- Specific AS events, such as those in CDK4, offer potential therapeutic targets for CRC.
- Findings provide a theoretical basis for developing targeted splicing therapies for colorectal cancer (CRC).
Related Concept Videos
02:18Alternative RNA Splicing
21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K
01:32RNA Splicing
56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
02:32Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
02:53Conservative Site-specific Recombination and Phase Variation
6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.0K
01:20Mismatch Repair
4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K