Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathway remodeling and adaptive evolution enable efficient co-utilization of glucose and xylose in <i>Escherichia coli</i>.

mBio·2026
Same author

A Novel Cold-Adapted and Heat-Resistant Alginate Lyase: Unveiling Its Characteristics and Mechanism of Thermostability.

Journal of agricultural and food chemistry·2026
Same author

Chlorogenic acid promotes macrophage phagocytosis through the METTL3-SIRPA axis in an m6A-dependent manner for colon cancer therapy.

Biochemical pharmacology·2026
Same author

Association Between Upper Airway Obstruction and Craniofacial Development and Orthodontic Outcomes in Children: A CBCT-Based Study.

The Journal of craniofacial surgery·2026
Same author

CILP2 exacerbates diabetes-induced muscle atrophy by over-activating skeletal muscle autophagy and inflammation via the P38 MAPK pathway.

International immunopharmacology·2026
Same author

Engineering of a LysG-derived arginine-specific biosensor for high-throughput screening of arginine overproducers in Corynebacterium glutamicum.

Biotechnology for biofuels and bioproducts·2026

Related Experiment Video

Updated: Jun 21, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.3K

High-Throughput Screening for Oligonucleotide Detection by ADE-OPI-MS.

Lingling Hu1,2, Zhidan Zhang3, Congyu Li4

  • 1College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.

Analytical Chemistry
|July 15, 2024
PubMed
Summary

A new high-throughput method using acoustic droplet ejection-open port interface-mass spectrometry (ADE-OPI-MS) accelerates oligonucleotide analysis. This breakthrough enhances terminal deoxynucleotide transferase (TdT) engineering for DNA synthesis.

More Related Videos

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.7K
Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

39.0K

Related Experiment Videos

Last Updated: Jun 21, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.3K
A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.7K
Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

39.0K

Area of Science:

  • Biochemistry and Molecular Biology
  • Analytical Chemistry
  • Biotechnology

Background:

  • Oligonucleotides are crucial in biomedicine, but high-throughput analysis remains a challenge.
  • Existing methods for oligonucleotide detection and analysis lack efficiency for large-scale studies.
  • Terminal deoxynucleotide transferase (TdT) plays a key role in DNA synthesis.

Purpose of the Study:

  • To develop a high-throughput analysis method for oligonucleotides.
  • To apply this method for engineering terminal deoxynucleotide transferase (TdT) with enhanced catalytic activity.
  • To improve the efficiency of DNA synthesis through enzyme engineering.

Main Methods:

  • Development of a high-throughput oligonucleotide analysis method using acoustic droplet ejection-open port interface-mass spectrometry (ADE-OPI-MS).
  • Application of the ADE-OPI-MS method to screen approximately 10,000 TdT mutants for enzymatic activity.
  • Integration of identified high-activity TdT variants to enhance catalytic function.

Main Results:

  • The developed ADE-OPI-MS method achieved an analysis rate of 3 seconds per sample, a 60-fold increase in efficiency over gel analysis.
  • Three novel TdT variants with significantly higher catalytic activities were identified.
  • The catalytic activity of TdT was improved approximately 4-fold by integrating the engineered mutants.

Conclusions:

  • A robust high-throughput screening method for oligonucleotide analysis was successfully established.
  • The engineered TdT variants provide a foundation for *ab initio* DNA synthesis.
  • This work opens avenues for broader applications of oligonucleotide analysis in biomedical research.